Sections (4μm) of formalin-fixed, paraffin-embedded tissues were deparaffinized with xylene and rehydrated. Serial sections were stained with H&E or Alcian Blue as described [17 (link)]. Immunohistochemistry was performed on a Leica BOND autostainer (Wetzlar, Germany). Slides were scanned in brightfield using the Aperio Digital Pathology System (Leica). Images were analyzed using the Tissue Classifier and Area Quantification modules of the HALO Image Analysis System v.3.0 (Indica labs, Albuquerque, NM). For immunofluorescence antigen retrieval was performed in a pressure cooker using 10mM Sodium citrate, pH 6.0. Primary antibodies were incubated overnight followed by incubation with secondary antibodies and TrueVIEW (SP-8400, Vector Laboratories, Burlingame, CA). Images were captured with a Nikon DSVi1 brightfield camera using NIS Elements 3.2 Basic Research Image software (Nikon Instruments Inc., Melville, NY).
Halo image analysis system v 3
Manufactured by Indica Labs
The HALO Image Analysis System v.3.0 is a digital pathology platform designed for high-throughput image analysis. It provides automated tools for segmentation, quantification, and visualization of histological samples. The system supports a wide range of whole-slide imaging formats and offers a user-friendly interface for image processing and data management.
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Lab products found in correlation
2 protocols using halo image analysis system v 3
1
Histopathological Analysis of Formalin-Fixed Tissues
2
Histopathological Analysis of Formalin-Fixed Tissues
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Sections (4μm) of formalin-fixed, paraffin-embedded tissues were deparaffinized with xylene and rehydrated. Serial sections were stained with H&E or Alcian Blue as described [17 (link)]. Immunohistochemistry was performed on a Leica BOND autostainer (Wetzlar, Germany). Slides were scanned in brightfield using the Aperio Digital Pathology System (Leica). Images were analyzed using the Tissue Classifier and Area Quantification modules of the HALO Image Analysis System v.3.0 (Indica labs, Albuquerque, NM). For immunofluorescence antigen retrieval was performed in a pressure cooker using 10mM Sodium citrate, pH 6.0. Primary antibodies were incubated overnight followed by incubation with secondary antibodies and TrueVIEW (SP-8400, Vector Laboratories, Burlingame, CA). Images were captured with a Nikon DSVi1 brightfield camera using NIS Elements 3.2 Basic Research Image software (Nikon Instruments Inc., Melville, NY).
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