Trpv1

Manufactured by Neuromics

Sourced in United States

TRPV1 is a transmembrane protein that functions as a cation channel. It is sensitive to vanilloids, protons, and heat stimuli, and plays a role in sensory perception and pain signaling.

Automatically generated - may contain errors

Lab products found in correlation

Lsm 700 confocal microscope, by Zeiss (2 mentions) P jnk, by Abcam (1 mentions) Pvdf membrane, by Bio-Rad (1 mentions) Erk1 2, by Abcam (1 mentions) Nf κb, by Abcam (1 mentions) Immedge hydrophobic barrier pen, by Vector Laboratories (1 mentions) Pla kit, by Merck Group (1 mentions) Dapi containing mounting medium, by Merck Group (1 mentions) Zen imaging software, by Zeiss (1 mentions) Cox 2, by Cell Signaling Technology (1 mentions) Ecl prime, by Cytiva (1 mentions) Protein assay kit, by Bio-Rad (1 mentions)

Spelling variants of this product

TRPV1 antibody (4 citations) Guinea pig anti-TRPV1 antibody (3 citations) Anti-TRPV1 (2 citations)

3 protocols using trpv1

Western Blot Analysis of MAPK Signaling Pathways

Check if the same lab product or an alternative is used in the 5 most similar protocols

DRG tissues were collected from mice and later disrupted in RIPA mixed with PMSF and protease inhibitor cocktail (1×) on ice. Total protein (20 μg) was separated by 10% SDS polyacrylamide gels and then transferred to PVDF membranes (Bio-Rad). After the membranes were blocked for 1 h at room temperature in Tris-buffered saline Tween-20 (TBST) containing 5% skim milk, followed by incubation with primary antibodies specific for p38MAPK (1:4000, CST, Danvers, MA, United States), p-p38MAPK (1:4000, CST), ERK1/2 (1:4000, Abcam, MA, United States), p-ERK1/2 (1:4000, CST), JNK (1:3000, Abcam), p-JNK (1:3000, Abcam), NF-κB (1:4000, Abcam), TRPV1 (1:2000, Neuromics, INA), and GAPDH (1:8000, CST) at 4°C overnight. After being washed with TBST three times, the membranes were incubated with secondary antibodies (1:10,000, Proteintech, WH, CHN) for 1 h at room temperature. After being washed with TBST three times, the antibodies were detected with ECL reagents (Tanon, SH, CHN). Immunoreactivity was detected by chemiluminescence. The results were quantified using ImageJ software.

Meng J., Qiu S., Zhang L., You M., Xing H, & Zhu J. (2022). Berberine Alleviate Cisplatin-Induced Peripheral Neuropathy by Modulating Inflammation Signal via TRPV1. Frontiers in Pharmacology, 12, 774795.

+ Open protocol

+ Expand

Proximity Ligation Assay for DRG Proteins

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

PLA was performed using PLA kits (Sigma) as previously described (19 (link)). Antibodies used were ANO1 (1:200; Santa Cruz or 1:500; Abcam), TRPV1 (1:500; Neuromics) and IP₃R1 (1:500; Calbiochem), CD71 (1:500, Santa Cruz). For anti-guinea pig antibodies (such as TRPV1), PLA ‘minus’ probes were manually conjugated onto an anti-guinea pig IgG antibody using a PLA conjugation kit. DRG cultures were prepared and plated on microscope slides (coated with poly-d-lysine and laminin) and permeabilized using acetone:methanol (1:1) on ice for 20 minutes and washed with PBS 3 times. Hydrophobic barriers were made on the slides using an ImmEdge hydrophobic barrier pen (Vector laboratories) to delimit reactions to ~1cm² and blocking was done using the PLA blocking reagent for 30 minutes in a humidified incubator (37°C). Primary antibodies were then applied and left at 4°C overnight. The following day, PLA probes were applied to the samples; signals were amplified and detected according to the manufacturer’s instructions; and slides were sealed with DAPI-containing mounting medium (Sigma). Samples were imaged using the Zeiss LSM700 confocal microscope. Green fluorescent puncta (0.5–1μm) were counted per cell using Zen imaging software (Zeiss).

Shah S., Carver C.M., Mullen P., Milne S., Lukacs V., Shapiro M.S, & Gamper N. (2020). Local Ca2+ signals couple activation of TRPV1 and ANO1 sensory ion channels. Science signaling, 13(629), eaaw7963.

+ Open protocol

+ Expand

Quantification of COX2 and TRPV1 Protein Levels

Check if the same lab product or an alternative is used in the 5 most similar protocols

Sciatic nerve and L4-L6 spinal cord were separated and homogenized using a homogenizer at 4 °C with lysis buffer (PRO-Prep™). Homogenized tissue centrifuged at 13,000 rpm for 10 min. The protein concentration was measured at 595 nm by adding Bio-Rad Protein Assay Kit (Bio-Rad Hercules, CA, USA). The prepared sample was subjected to electrophoresis and nitrocellulose membrane protein transfer and COX2 (1:1000, Cell Signaling, Danvers, MA, USA) and TRPV1 (1:1000, Neuromics, Edina, MN, USA) primary antibody were reacted at 4 °C for 24 h, reacted with secondary antibody for 2 h, and then ECL prime (Amersham Pharmacia Biotech, Buckinghamshire, UK). The degree of expression was measured using Image J software.

Kang M.S, & Hyun K.Y. (2020). Antinociceptive and Anti-Inflammatory Effects of Nypa fruticans Wurmb by Suppressing TRPV1 in the Sciatic Neuropathies. Nutrients, 12(1), 135.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!