Section 4 m of each case are used to perform HPV RNA ISH test. Detection of high-risk-HPV E6/E7 mRNA was performed using Ready-to-use reagents from RNAscope 2.5 LS Reagent Kit-BROWN and the HPV-HR18 probe cocktail (Advanced Cell Diagnostics) that were loaded onto the Leica Biosystems’ BOND RX Research Advanced Staining System according to the user manual (Doc. No. 322,100-USM). The slides were independently evaluated by three separate observers. Ubiquitin C and dapB were used as positive and negative controls, respectively. A positive HPV ISH test result was defined as positive if any of the malignant cells showed brown punctate dot-like nuclear and/or cytoplasmatic positivity [27 ].
Bond rx research advanced staining system
Manufactured by Leica
The BOND RX Research Advanced Staining System is a fully automated staining platform designed for immunohistochemistry and in situ hybridization assays. It offers reproducible, high-quality staining results with precise control over the staining process. The system is capable of performing a wide range of staining protocols, making it a versatile tool for researchers in the field of tissue analysis and diagnostics.
Automatically generated - may contain errors
3 protocols using bond rx research advanced staining system
1
HPV RNA ISH Analysis Protocol
2
HPV RNA In Situ Hybridization Protocol
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Sections 4 μ mof each case are used to perform HPV RNA ISH test. Detection of high-risk-HPV E6/E7 mRNA was performed using Ready-to-use reagents from RNAscope 2.5 LS Reagent Kit-BROWN and the HPV-HR18 probe cocktail (Advanced Cell Diagnostics) that were loaded onto the Leica Biosystems’ BOND RX Research Advanced Staining System according to the user manual (Doc. No. 322100-USM). The slides were independently evaluated by three separate observers (FZM, AR and RF) . Ubiquitin C and dapB were used as positive and negative controls, respectively. A positive HPV ISH test result was defined as positive if any of the malignant cells showed brown punctate dot-like nuclear and/or cytoplasmatic positivity [21 (link), 22 (link)].
3
HPV RNA ISH Detection in Malignant Cells
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A section of 4-μm thickness from each case was used to perform the HPV RNA ISH test. Detection of high-risk HPV E6/E7 mRNA was performed using ready-to-use reagents from RNA scope 2.5 LS Reagent Kit-BROWN and the HPV-HR18 probe cocktail (Advanced Cell Diagnostics) that were loaded onto the Leica Biosystems’ BOND RX Research Advanced Staining System according to the user manual (Doc. No. 322,100-USM). The slides were independently evaluated by two separate observers.
Ubiquitin C and dapB were used as positive and negative controls, respectively. A positive HPV ISH test result was defined as positive if any of the malignant cells showed brown punctate dot-like nuclear and/or cytoplasmic positivity (19 (link)).
Ubiquitin C and dapB were used as positive and negative controls, respectively. A positive HPV ISH test result was defined as positive if any of the malignant cells showed brown punctate dot-like nuclear and/or cytoplasmic positivity (19 (link)).
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