The coverslips or slices were postfixed with 4% paraformaldehyde for 24 hours at 4°C, washed three times with PBS, blocked for 2 hours with 0.1% Triton X-100 and 1% BSA at 25°C, and subjected to antigen repair with sodium citrate at 98°C for 20 minutes. The samples were incubated with primary antibodies NeuN (1 : 500, ET1602-12, HuaBio, Hangzhou, China), Tuj-1 (1 : 500, M0805-8, HuaBio, Hangzhou, China), Iba-1 (1 : 500, RT1316, HuaBio, Hangzhou, China), and GFAP (1 : 500, EM140707, HuaBio, Hangzhou, China) overnight at 4°C, washed three times with PBS, and incubated with secondary antibodies (1 : 500, A21121, Invitrogen, USA) for 2 hours at room temperature. For each clinical or animal sample, all immunofluorescence-positive cells from 3 random inconsecutive slices were picked.
M0805 8
Manufactured by Huabio
Sourced in China
The M0805-8 is a laboratory equipment that serves as a magnetic bead separator. It is designed to efficiently separate magnetic beads from liquid samples, which is a common step in various biochemical and molecular biology applications.
Automatically generated - may contain errors
Lab products found in correlation
A21121, by Thermo Fisher Scientific (1 mentions)
Rt1316, by Huabio (1 mentions)
Alexa fluor 594 goat anti mouse igg, by Thermo Fisher Scientific (1 mentions)
Sc 390572, by Santa Cruz Biotechnology (1 mentions)
Sc 57053, by Santa Cruz Biotechnology (1 mentions)
Fv1000, by Olympus (1 mentions)
Bl539a, by Biosharp (1 mentions)
2 protocols using m0805 8
1
Immunohistochemical Labeling of Neural Cell Types
2
Immunofluorescence Imaging of DRG Neurons
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Immunofluorescence was performed as described in previous reports.28 (link) Briefly, L3-L4 DRGs were rapidly detached from mice perfused with 4% paraformaldehyde (BL539 A, Biosharp) and post-fixed with 4% paraformaldehyde for 2 h, followed by dehydration in 5%, 15%, 30% sucrose gradients, respectively, and sectioned at a thickness of 12 mm on a cryostat. After blocking at room temperature for 1 h and washing with PBST (containing 0.04% Triton), the slices were incubated with anti-rabbit ETS1 antibody (1:1000; A13302, ABclonal), mouse anti-glutamine synthetase (GS; 1:800; EM1902-39, Hua Bio), mouse anti-β-tubulin (1:800; M0805-8, Hua Bio), mouse anti-CGRP (1:50; SC57053, Santa Cruz), mouse anti-P2X3 (1:100; SC390572, Santa Cruz), or mouse anti-NF200 (1:100; 60,331-1-Ig, Proteintech) at 4°C overnight. After washing with PBST, slices were finally incubated with fluorescent-conjugated secondary antibodies Alexa FluorTM 488 goat anti-rabbit IgG (A21206, Molecular Probes) and Alexa Fluor™ 594 goat anti-mouse IgG (A10036, Molecular Probes). The sections were mounted on a mounting medium with DAPI (0100-20, Southern Biotech) and scanned using a high-resolution digital confocal microscope (Olympus FV1000, Japan).
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