Cells were lysed in prechilled RIPA buffer containing phosphatase inhibitors, protease inhibitors and PMSF. The protein extracts were loaded on each line of a 10% SDS-PAGE gel and transferred to polyvinylidene fluoride (PVDF) membranes (Millipore, Darmstadt, Germany). The membranes were blocked in 5% skimmed milk in 1 × PBS-T (0.5% Tween-20) and incubated overnight at 4°C with the following primary antibodies: anti-SLC25A21 (1:500; Affinity Biosciences), anti-cytochrome C (1:500; Affinity Biosciences), anti-caspase-9 (1:800; Affinity Biosciences), anti-cleaved caspase-9 (1:800; Affinity Biosciences), anti-caspase-3 (1:800; Affinity Biosciences), and anti-cleaved caspase-3 (1:800; Affinity Biosciences). Anti-tubulin (1:1000; Proteintech Group, Wuhan, China) was used as protein-loading control. Blots were incubated with HRP-conjugated secondary antibodies for 1 h at room temperature, and visualized with ECL Western Blotting Substrate (ThermoFisher Scientific).
Anti cytochrome c
Manufactured by Affinity Biosciences
Anti-cytochrome C is a laboratory reagent used to detect and quantify the presence of cytochrome C, a protein involved in cellular respiration and apoptosis. It functions by specifically binding to cytochrome C, allowing for its identification and measurement in various biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Anti caspase 3, by Affinity Biosciences (2 mentions)
Anti tubulin, by Proteintech (2 mentions)
Anti cleaved caspase 3, by Affinity Biosciences (2 mentions)
Ecl western blotting substrate, by Thermo Fisher Scientific (2 mentions)
Pvdf membrane, by Merck Group (2 mentions)
Anti slc25a21, by Affinity Biosciences (2 mentions)
Anti caspase 9, by Affinity Biosciences (2 mentions)
Anti cleaved caspase 9, by Affinity Biosciences (2 mentions)
2 protocols using anti cytochrome c
1
Western Blot Analysis of Apoptosis Markers
2
Western Blot Analysis of Apoptosis Markers
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Cells were lysed in prechilled RIPA buffer containing phosphatase inhibitors, protease inhibitors and PMSF. The protein extracts were loaded on each line of a 10% SDS-PAGE gel and transferred to polyvinylidene fluoride (PVDF) membranes (Millipore, Darmstadt, Germany). The membranes were blocked in 5% skimmed milk in 1 × PBS-T (0.5% Tween-20) and incubated overnight at 4 °C with the following primary antibodies: anti-SLC25A21 (1:500; Affinity Biosciences), anti-cytochrome C (1:500; Affinity Biosciences), anti-caspase-9 (1:800; Affinity Biosciences), anti-cleaved caspase-9 (1:800; Affinity Biosciences), anti-caspase-3 (1:800; Affinity Biosciences), and anti-cleaved caspase-3 (1:800; Affinity Biosciences). Anti-tubulin (1:1000; Proteintech Group, Wuhan, China) was used as protein-loading control. Blots were incubated with HRP-conjugated secondary antibodies for 1 h at room temperature, and visualized with ECL Western Blotting Substrate (ThermoFisher Scientific).
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