Human rheumatoid synovial fibroblasts were treated with 5 μM PF-562,271 (Symansis, Timaru, New Zealand), 5 μM PF-573,228 (Tocris Bioscience, Bristol, UK) or an equal volume of dimethyl sulfoxide (DMSO) for 10 minutes at room temperature before and also during migration, invasion or degradation assays. The final concentration of DMSO was 0.5% or less for all experiments. Dosing of drugs and DMSO was based on previous studies [24 (link)] and confirmed not to alter cell metabolism, viability and function (data not shown). TNF+CreER+FAKf/f murine synovial fibroblasts were treated with 1 μM (Z)-4-OH tamoxifen (Sigma-Aldrich, St Louis, MO, USA) in 95% ethanol or vehicle control (95% ethanol) for 4 days before experiments.
Z 4 oh tamoxifen
Manufactured by Merck Group
Sourced in United States, Germany
The (Z)-4-OH tamoxifen is a laboratory research tool. It is a metabolite of the drug tamoxifen. (Z)-4-OH tamoxifen is used in scientific research settings to study the properties and effects of tamoxifen.
Automatically generated - may contain errors
Lab products found in correlation
Pf573228, by Bio-Techne (1 mentions)
Tamoxifen, by Innovative Research (1 mentions)
Paxilline, by Merck Group (1 mentions)
Progesterone, by Merck Group (1 mentions)
Z endoxifen, by Merck Group (1 mentions)
E2 bsa, by Merck Group (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Testosterone, by Merck Group (1 mentions)
Tamoxifen, by Merck Group (1 mentions)
Fulvestrant, by Merck Group (1 mentions)
Carprofen, by Zoetis (1 mentions)
2 protocols using z 4 oh tamoxifen
1
Fibroblast Migration and Invasion Assays
2
Preparation of Hormone and Compound Solutions
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Tamoxifen-containing and placebo pellets were supplied by Innovative Research of America, Sarasota, USA. Metamizole was supplied by 1A Pharma (Oberhaching, Germany) and carprofen by Zoetis (Delemont, Switzerland). Stock solutions (1 mM) of E2, progesterone, testosterone, Tamoxifen, (Z)-endoxifen, (Z)-4-OHTamoxifen, fulvestrant (all from Sigma-Aldrich, Taufkirchen, Germany) were prepared in DMSO (Sigma-Aldrich). The stock solution of E2-BSA (Sigma-Aldrich) was prepared in PBS. Stock solutions were serially diluted to the final concentrations applied, as indicated in Figures 5 and6. Paxilline (Sigma-Aldrich) was dissolved in DMSO to provide a stock solution (10 mM), aliquoted and stored frozen.
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