Transwell 0.4 μm pore size filters

Manufactured by Corning

Transwell 0.4‐μm pore‐size filters are a type of lab equipment used for cell culture applications. They feature a permeable membrane with 0.4‐micron pores, which allows the passage of small molecules and solutes while retaining cells on the membrane surface.

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Lab products found in correlation

Evom voltmeter, by World Precision Instruments (1 mentions) Endohm 12, by World Precision Instruments (1 mentions)

2 protocols using transwell 0.4 μm pore size filters

Transepithelial Electrical Resistance Measurement

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The cells were cultured to confluence in the inner chambers of 12‐mm Transwell 0.4‐μm pore‐size filters (Corning Life Science). Transepithelial electrical resistance (TEER) was measured using an EVOM voltmeter with an ENDOHM‐12 (World Precision Instruments, Sarasota, FL) on a heating plate (Fine, Tokyo, Japan) and was monitored using a cellZscope (nanoAnalytics, Münster Germany), a computer controlled automated multiwell device (12 wells). The values were expressed in standard units of ohms per square centimeter and presented as the mean ± SD of triplicate experiments. For calculation, the resistance of blank filters was subtracted from that of filters covered with cells.

Kono T., Kondoh M., Kyuno D., Ito T., Kimura Y., Imamura M., Kohno T., Konno T., Furuhata T., Sawada N., Hirata K, & Kojima T. (2015). Claudin‐4 binder C‐CPE 194 enhances effects of anticancer agents on pancreatic cancer cell lines via a MAPK pathway. Pharmacology Research & Perspectives, 3(6), e00196.

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Transepithelial Electrical Resistance Measurement

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Cells were cultured to confluence on the inner chambers of 12-mm Transwell 0.4-μm pore-size filters (Corning Life Science). Transepithelial electrical resistance (TER) was monitored using a cellZscope (nanoAnalytics, Germany), a computer controlled automated multi-well device (12 wells). The values are expressed in standard units of ohms per square centimeter and presented as the mean ± SD of triplicate experiments.

Nomura K., Obata K., Keira T., Miyata R., Hirakawa S., Takano K.I., Kohno T., Sawada N., Himi T, & Kojima T. (2014). Pseudomonas aeruginosa elastase causes transient disruption of tight junctions and downregulation of PAR-2 in human nasal epithelial cells. Respiratory Research, 15(1), 21.

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