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Genjet lipofection reagent

Manufactured by SignaGen
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GenJet Lipofection reagent is a cationic lipid-based transfection agent designed for the delivery of nucleic acids, including plasmid DNA, siRNA, and mRNA, into a wide range of cell types. The reagent forms lipoplexes with the nucleic acids, facilitating their uptake and expression within the target cells.

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Lab products found in correlation

Lipofectamine 2000, by Thermo Fisher Scientific (3 mentions) Pgl4.74 hrluc tk, by Promega (1 mentions) Gfp rac1, by Addgene (1 mentions) Tcf reporter plasmid kit, by Merck Group (1 mentions) Dual luciferase reporter assay system, by Promega (1 mentions)

Close spelling variants of this product

GenJet (38 citations) GenJet reagent (9 citations)

2 protocols using genjet lipofection reagent

1

Plasmid Constructs for Wnt Signaling

Cited 1 time
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The UBC-Fzd7-Flag (Fzd7-Flag; backbone: pLenti-III-UBC), CMV-Fzd7-EYFP (Fzd7-YFP; backbone: pEYFP-N1), CMV-Fzd3-EYFP (Fzd3-YFP; backbone: pEYFP-N1), CMV-EYFP (YFP; backbone: pEYFP-N1), CMV-EGFP-Rac1-wt (GFP-Rac1, 12980; Addgene; backbone: pcDNA3-EGFP), CMV-EGFP-Rac1-T17N (Rac1-DN, 12982; Addgene; backbone: pcDNA3-EGFP), CMV-EGFP-RhoA-T19N (RhoA-DN, 12967; Addgene; backbone: pcDNA3-EGFP), and CMV-EGFP-Cdc42-T17N (Cdc42-DN, 12976; Addgene; backbone: pcDNA3-EGFP) constructs have been described previously (Subauste et al., 2000 (link); von Maltzahn et al., 2011 (link); Bentzinger et al., 2013a (link)). The Fzd7-tdtomato plasmid was generated by replacing the C-terminal YFP in Fzd7-YFP with tdTomato. For TOP-flash and FOP-flash the TCF reporter plasmid kit (17-285; EMD Millipore) was used with the dual-luciferase reporter assay system (E1960; Promega). For normalization, pGL4.74[hRluc/TK] (E6921; Promega) was cotransfected. For TOP-flash and FOP-flash assays the cells were transfected with the GenJet Lipofection reagent (SL100499; SignaGen). Otherwise, Lipofectamine 2000 (11668019; Life Technologies) was used for all transfections.
Bentzinger C.F., von Maltzahn J., Dumont N.A., Stark D.A., Wang Y.X., Nhan K., Frenette J., Cornelison D, & Rudnicki M.A. (2014). Wnt7a stimulates myogenic stem cell motility and engraftment resulting in improved muscle strength. The Journal of Cell Biology, 205(1), 97-111.
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2

Transduction and Gene Editing Protocols

Cited 1 time
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HEK293 cells were transfected with rCAV1.2 WT (150 ng), pcDNA3.1 α2δ1 (75 ng), pcDNA3.1 β2 α (75 ng) and Cherry-C1 (20 ng) kindly provided by Dr. R.W. Tsien (NYU) using Lipofectamine 2000 (Thermo Fisher Scientific). Cells were used for electrophysiology 24–48 h after transfection. To produce pseudotyped retrovirus for transduction of T cells, Plat E cells were transfected with retroviral expression plasmids encoding shRNAs (pLMPd-Amt, pLMPd-GFP)81 (link) and small guide RNAs for CRISPR/Cas9 gene editing (pMIR-Amt, pMRI-GFP82 (link). Plat E cells were cotransfected with the ecotropic packaging vector pCL-Eco using GenJet lipofection reagent (SignaGen, SL100489). Retroviral supernatant was collected 36 and 60 h after transfection.
Erdogmus S., Concepcion A.R., Yamashita M., Sidhu I., Tao A.Y., Li W., Rocha P.P., Huang B., Garippa R., Lee B., Lee A., Hell J.W., Lewis R.S., Prakriya M, & Feske S. (2022). Cavβ1 regulates T cell expansion and apoptosis independently of voltage-gated Ca2+ channel function. Nature Communications, 13, 2033.
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