Lamin b2

Manufactured by Proteintech

Lamin B2 is a protein that is a component of the nuclear envelope. It plays a structural role in maintaining the integrity and organization of the nuclear lamina.

Automatically generated - may contain errors

Lab products found in correlation

Odyssey fc, by LI COR (2 mentions) Irdye 680rd goat anti rabbit igg, by LI COR (2 mentions) Gapdh, by Merck Group (2 mentions) Lamin b1, by Proteintech (2 mentions) Pthr18 ser19 mlc2, by Cell Signaling Technology (2 mentions) Irdye 800rd goat anti mouse igg, by LI COR (2 mentions) Lamin a c, by Proteintech (1 mentions) Lds buffer 1x, by Thermo Fisher Scientific (1 mentions)

2 protocols using lamin b2

Western Blot Analysis of Melanoma Cells

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Melanoma cells were seeded at a density of 6 × 10⁵ cells ml⁻¹ in 12-well plates and lysed the next day with LDS buffer 1× (Life Technologies). Lysates were denatured at 95 °C for 5 min and sonicated. SDS–PAGE and western blotting were performed using standard procedures. Membranes were visualized and bands quantified using an Odyssey Fc (LI-COR). Loading controls were run on the same blot. Individual 680 and 800 channels were co-visualized in greyscale on the same image. Primary antibodies were: LAP1 (1:1,000; #21459-1-AP), Lamin A/C (1:1,000, #10298-1-AP), Lamin B1 (1:1,000, #12987-1-AP), Lamin B2 (1:1,000, #10895-1-AP) from Proteintech; pThr18/Ser19-MLC2 (1:750, #3674), MLC2 (1:750, #3672) from Cell Signaling Technology; GAPDH (1:10,000, #MAB374) from Merck. Secondary antibodies were: IRDye 680RD goat anti-rabbit IgG (1:10,000, #925-68071) and IRDye 800RD goat anti-mouse IgG (1:10,000, #925-32210) from LI-COR.

Jung-Garcia Y., Maiques O., Monger J., Rodriguez-Hernandez I., Fanshawe B., Domart M.C., Renshaw M.J., Marti R.M., Matias-Guiu X., Collinson L.M., Sanz-Moreno V, & Carlton J.G. (2023). LAP1 supports nuclear adaptability during constrained melanoma cell migration and invasion. Nature Cell Biology, 25(1), 108-119.

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Quantification of Melanoma Cell Proteins

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Melanoma cells were seeded at a density of 6x10⁵ cells/ml in 12-well plates and lysed the next day with LDS buffer 1X (Life Technologies). Lysates were denatured at 95°C for 5 minutes and sonicated. SDS-PAGE and western blotting was performed using standard procedures. Membranes were visualised and bands quantified using an Odyssey Fc (LI-COR). Loading controls were run on the same blot. Individual 680 and 800 channels were co-visualised in greyscale on the same image. Primary antibodies were: LAP1 (1:1000; #21459-1-AP), Lamin A/C (1:1000, #10298-1-AP), Lamin B1 (1:1000, #12987-1-AP), Lamin B2 (1:1000, #10895-1-AP) from Proteintech; pThr18/Ser19-MLC2 (1:750, #3674), MLC2 (1:750, #3672) from Cell Signalling Technology; GAPDH (1:10,000, #MAB374) from Merck. Secondary antibodies were: IRDye 680RD goat anti-rabbit IgG (1:10,000, #925-68071) and IRDye 800RD goat anti-mouse IgG (1:10,000, #925-32210) from LI-COR.

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