Axio observer z 1 spinning disk confocal

Manufactured by Zeiss

The Zeiss Axio Observer Z.1 Spinning Disk Confocal is a high-performance microscope system designed for advanced live-cell imaging. It utilizes a spinning disk confocal scanning technology to provide fast, high-resolution imaging of fluorescently labeled samples.

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Lab products found in correlation

A4503, by Merck Group (3 mentions) Prolong antifade mounting solution with dapi, by Thermo Fisher Scientific (3 mentions) Superfrost slide, by Thermo Fisher Scientific (3 mentions) T8787, by Merck Group (3 mentions) Imagexpress micro confocal high content imaging system, by Molecular Devices (3 mentions) Phosphate buffered saline (pbs), by Corning (1 mentions)

Spelling variants of this product

Axio Observer (1225 citations) Axio Observer Z (6 citations) Axio Observer Z.1 Spinning Disk Confocal Microscope (2 citations)

3 protocols using axio observer z 1 spinning disk confocal

Immunofluorescence Staining of Cultured Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Infected and mock-treated cell cultures in coverslips were washed with
Phosphate Buffered Solution (PBS, Corning) and fixed in 4% paraformaldehyde
(PFA) overnight, followed by blocking and permeabilization with 0.1% Triton-X
100 (T8787, Sigma) and 5% BSA (A4503, Sigma) for one hour at RT. Antibody
dilution buffer (Ab buffer) was comprised of PBS supplemented with 0.1% Triton-X
100 and 1% BSA. Samples were incubated with primary antibodies overnight at
4°C (table S2),
followed by 3 washes with PBS and incubation with fluorescent-conjugated
secondary antibodies at 1:250 in Ab buffer for 1 hour at RT (table S2). Coverslips were mounted
onto SuperFrost Slides (FisherBrand, 12-550-15) with ProLong Antifade mounting
solution with DAPI (Invitrogen, P36931). Images were acquired with a Zeiss Axio
Observer Z.1 Spinning Disk Confocal (Carl Zeiss) or with an ImageXpress Micro
Confocal High-Content Imaging System (Molecular Devices) and processed using
ZenBlue and ImageJ.

Perez-Bermejo J.A., Kang S., Rockwood S.J., Simoneau C.R., Joy D.A., Silva A.C., Ramadoss G.N., Flanigan W.R., Fozouni P., Li H., Chen P.Y., Nakamura K., Whitman J.D., Hanson P.J., McManus B.M., Ott M., Conklin B.R, & McDevitt T.C. (2021). SARS-CoV-2 infection of human iPSC-derived cardiac cells reflects cytopathic features in hearts of patients with COVID-19. Science Translational Medicine.

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Immunofluorescence Staining of Cultured Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Infected and mock-treated cell cultures in coverslips were washed with Phosphate Buffered Solution (PBS) and fixed in 4% paraformaldehyde (PFA) overnight, followed by blocking and permeabilization with 0.1% Triton-X 100 (T8787, Sigma) and 5% BSA (A4503, Sigma) for one hour at RT. Antibody dilution buffer (Ab buffer) was comprised of PBS supplemented with 0.1% Triton-X 100 and 1% BSA. Samples were incubated with primary antibodies overnight at 4°C (Table 2), followed by 3 washes with PBS and incubation with fluorescent-conjugated secondary antibodies at 1:250 in Ab buffer for 1 hour at RT (Table 2). Coverslips were mounted onto SuperFrost Slides (FisherBrand, 12-550-15) with ProLong Antifade mounting solution with DAPI (Invitrogen, P36931. Images were acquired with a Zeiss Axio Observer Z.1 Spinning Disk Confocal (Carl Zeiss) or with an ImageXpress Micro Confocal High-Content Imaging System (Molecular Devices) and processed using ZenBlue and ImageJ.

Pérez-Bermejo J.A., Kang S., Rockwood S.J., Simoneau C.R., Joy D.A., Ramadoss G.N., Silva A.C., Flanigan W.R., Li H., Nakamura K., Whitman J.D., Ott M., Conklin B.R, & McDevitt T.C. (2020). SARS-CoV-2 infection of human iPSC-derived cardiac cells predicts novel cytopathic features in hearts of COVID-19 patients. bioRxiv.

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Immunofluorescence Staining of Cell Cultures

Check if the same lab product or an alternative is used in the 5 most similar protocols

Infected and mock-treated cell cultures in coverslips were washed with Phosphate Buffered Solution (PBS, Corning) and fixed in 4% paraformaldehyde (PFA) overnight, followed by blocking and permeabilization with 0.1% Triton-X 100 (T8787, Sigma) and 5% BSA (A4503, Sigma) for one hour at RT. Antibody dilution buffer (Ab buffer) was comprised of PBS supplemented with 0.1% Triton-X 100 and 1% BSA. Samples were incubated with primary antibodies overnight at 4°C (table S2), followed by 3 washes with PBS and incubation with fluorescent-conjugated secondary antibodies at 1:250 in Ab buffer for 1 hour at RT (table S2). Coverslips were mounted onto SuperFrost Slides (FisherBrand, 12-550-15) with ProLong Antifade mounting solution with DAPI (Invitrogen, P36931). Images were acquired with a Zeiss Axio Observer Z.1 Spinning Disk Confocal (Carl Zeiss) or with an ImageXpress Micro Confocal High-Content Imaging System (Molecular Devices) and processed using ZenBlue and ImageJ.

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