Anti acetyl α tubulin lys 40 antibody

Mouse monoclonal anti-α-tubulin FITC antibody, anti-phalloidin-TRITC antibody, anti-acetyl-α-tubulin (Lys-40) antibody and lens culinaris agglu-tinin (LCA)-FITC were purchased from Sigma (St. Louis, MO, USA); rabbit polyclonal anti-human ovastacin antibody was obtained from Dr. Jurrien Dean (NIH); FITC-conjugated goat anti-mouse IgG (H + L) and TRITC-conjugated goat anti-mouse IgG (H + L) were purchased from Zhongshan Golden Bridge Biotechnology Co., LTD (Beijing, China).

Antibodies were as follows: Mouse monoclonal anti-α-tubulin antibody (1:200, Sigma, St. Louis, MO, United States, #F2168); anti-acetyl-α-tubulin (Lys-40) antibody (1:100, Sigma #T7451); phalloidin-TRITC (1:200, Sigma, #P1951); rabbit monoclonal to gamma H2A.X (gH2A.X) (1:200, Abcam, Cambridge, United Kingdom, #ab81299); rabbit polyclonal anti-di-methyl-histone H3 (Lys4) (H3K4me2) antibody (1:200, Cell Signaling Technology, Danvers, MA, United States); rabbit polyclonal anti-tri-methyl-histone H3 (Lys4) (H3K4me3) antibody (1:200, Cell Signaling Technology, United States, #C42D8); rabbit polyclonal anti-trimethyl-histone H3 (Lys36) (H3K36me3) antibody (1:200, ABclonal, United States, #A2366). Alexa Fluor 488 goat anti-mouse antibody (1:200, Invitrogen #A11126, Carlsbad, CA, United States); MitoTracker Red CMXRos (Thermo Fisher Scientific, #7512, Waltham, MA, United States); Alexa Fluor 594 goat anti-rabbit antibody (Invitrogen, Carlsbad, CA, United States). Microcystin-LR was purchased from APExBIO Technology (Houston, TX, United States, #B3698); VC was purchased from Sigma (Shanghai, China, #A7506). The basic maturation culture medium used was tissue culture medium (TCM-199; Sigma). Phosphate-buffered saline (PBS) was purchased from Life Technologies (Invitrogen).

Mouse monoclonal anti-α-tubulin FITC antibody, anti-phalloidin-TRITC antibody, anti-acetyl-α-tubulin (Lys-40) antibody and lens culinaris agglutinin (LCA)-FITC were purchased from Sigma (St. Louis, MO, USA); FITC-conjugated goat anti-mouse IgG (H + L) and TRITC-conjugated goat anti-mouse IgG (H + L) were purchased from Zhongshan Golden Bridge Biotechnology Co., LTD (Beijing, China). DDP was purchased from Beijing Solarbio Science and Technology Co., LTD. (Beijing, China). TP was from Selleck Chemicals (Houston, TX, USA).