The human CCA cell line KMBC, HCCC9810 and RBE were obtained from Shanghai Bioleaf Biotech Co., Ltd. (Shanghai, China). QBC939 cell line was given as a present by Professor SG Wang, from the Third Military Medical University of China. The normal human intrahepatic biliary cell line (HIBEpiC) was obtained from ScienCell Research Laboratories (Carlsbad, CA). CCA cell lines were cultured in RPMI1640 or DMEM supplemented with 10% fetal bovine serum (FBS). LY294002, CHX and IGF1 was purchased from Sigma-Aldrich. IGF1 ELISA Kit was obtained from Abcam.
Igf1 elisa kit
Manufactured by Abcam
Sourced in United States
The IGF1 ELISA Kit is a quantitative sandwich enzyme immunoassay designed for the measurement of IGF1 levels in human serum, plasma, and cell culture supernatants. It utilizes a specific antibody coated on a microplate to capture IGF1, which is then detected using a detection antibody. The kit provides a reliable and accurate method for the determination of IGF1 concentrations.
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3 protocols using igf1 elisa kit
1
CCA Cell Line Cultivation and Characterization
2
Measuring Protein and Enzyme Profiles in C2C12 Cells
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To measure protein expression and enzyme activity in vitro, C2C12 myoblast cells were treated with test compounds in differentiation media for 24 h. The expressions of IGF-1 and collagen 1 in the culture medium were measured using an IGF-1 ELISA kit (Abcam, Cambridge, MA, USA) and a collagen type I ELISA kit (Chondrex Inc., Redmond, WA, USA), respectively, according to the manufacturers’ instructions. For measuring the energy-metabolism related enzyme activities and metabolites, cell were incubated with serum-free medium containing test samples for 16 h. After washing with ice-cold PBS three times, cells were collected and lysed with assay buffer. Cell lysates were centrifuged (12,000 g, 10 min). The supernatant was collected and creatine kinase (CK) and lactate dehydrogenase (LDH) activities were measured by appropriate activity assay kits (Abcam), after filtering the supernatant with a 10kDa spin column (Abcam). ATP and lactate concentrations in the supernatant were also measured using an ATP assay kit and a lactate assay kit (Abcam), respectively.
3
Quantifying Secreted IGF-1 from MSCs
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The supernatant from cultured MSCs was collected, and the levels of secreted IGF-1 were quantified with an IGF-1 ELISA Kit (Abcam, USA).
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