Representative hematoxylin and eosin-stained sections on glass slides containing prominent intratumoral regions from the 152 CCRCC specimens were chosen. Two cores were obtained from each representative paraffin block and transferred to recipient TMA blocks. Immunohistochemical staining was performed on the TMA blocks. Anti-MYOF monoclonal antibody (1:100 dilution, #ab76746, Abcam, UK), anti-VEGFR2 polyclonal antibody (1:50 dilution, #RB-1526-P1, Thermo Fisher Scientific, USA), and anti-CD31 monoclonal antibody (1:100 dilution, JC70, Cell Marque, USA) were used as the primary antibodies. The absolute number of positive CD31 endothelial cells was automatically counted using the Genie analysis tool (Leica Biosystems, Wetzlar, Germany).
Ab76746
Manufactured by Abcam
Sourced in United Kingdom
Ab76746 is an antibody that targets a specific protein. This product is suitable for research use.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using ab76746
1
Quantifying Angiogenesis in Renal Cell Carcinoma
2
Immunohistochemical Analysis of Myoferlin
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To investigate protein expression, immunohistochemical staining was performed on 4 μm sections from the TMA blocks. The sections were deparaffinized, rehydrated, and incubated in 3% hydrogen peroxide. After heating in 10 mM citrate buffer (pH 6.0), the slides were incubated with a mouse monoclonal primary myoferlin antibody (1:100, 7D2, ab76746, Abcam, Cambridge, UK). 3,3'-Diaminobenzidine staining and counterstaining of hematoxylin were carried out for visualization. The expression of myoferlin in the tumor cells was graded from negative to weak (+1), moderate (+2), and strong (+3) positivity by two experienced pathologists. Normal thyroid follicular cells were used as the internal control. If tumor cells exhibited equivalent intensity of myoferlin expression compared to normal follicular cells, they were graded as moderate positive. Similarly, tumor cells that exhibited stronger or weaker expression were graded as strong and weak positive, respectively.
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