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Anti phospho pdgfrβ

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Anti-phospho-PDGFRβ is a laboratory reagent that detects the phosphorylated form of the platelet-derived growth factor receptor beta (PDGFRβ) protein. This antibody can be used to monitor the activation status of PDGFRβ in various experimental systems.

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Lab products found in correlation

Anti phospho akt, by Cell Signaling Technology (3 mentions) Anti pdgfrβ, by Cell Signaling Technology (3 mentions) Anti vinculin n 19, by Santa Cruz Biotechnology (2 mentions) Anti akt, by Cell Signaling Technology (2 mentions) Anti parp, by Cell Signaling Technology (2 mentions) Anti phospho pdk1, by Cell Signaling Technology (1 mentions) Anti pdk1, by Cell Signaling Technology (1 mentions) Anti igf1rβ, by Cell Signaling Technology (1 mentions) Anti s6k, by Cell Signaling Technology (1 mentions) Anti phospho igf 1rβ, by Cell Signaling Technology (1 mentions) Anti phospho mtor, by Cell Signaling Technology (1 mentions) Anti tsc2, by Cell Signaling Technology (1 mentions) Anti egfr, by Merck Group (1 mentions) Anti irs1, by Cell Signaling Technology (1 mentions) Anti met, by Merck Group (1 mentions) Anti phospho met, by Cell Signaling Technology (1 mentions) Anti pten, by Cell Signaling Technology (1 mentions) Anti mtor, by Cell Signaling Technology (1 mentions) Anti phospho 4ebp1, by Cell Signaling Technology (1 mentions) Anti tsc1, by Cell Signaling Technology (1 mentions)

Spelling variants of this product

PDGFRβ (64 citations) Anti-PDGFRβ (40 citations)

5 protocols using anti phospho pdgfrβ

1

Western Blot Analysis of PDGFR-β and Akt

Cited 1 time
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Cell lysates preparation and Western blot analyses were performed as previously reported 31 (link). Filters were probed with the indicated primary antibodies: anti-phospho-PDGFRβ (Tyr771, indicated as pPDGFRβ), anti-PDGFRβ, anti-phospho-Akt (Ser473, indicated as pAkt) (Cell Signaling Technology Inc.) and anti-vinculin (N-19) (Santa Cruz Biotechnology, Santa Cruz, CA).
Camorani S., Hill B.S., Collina F., Gargiulo S., Napolitano M., Cantile M., Di Bonito M., Botti G., Fedele M., Zannetti A, & Cerchia L. (2018). Targeted imaging and inhibition of triple-negative breast cancer metastases by a PDGFRβ aptamer. Theranostics, 8(18), 5178-5199.
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2

Antibody Sourcing and Characterization

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Anti-NDRG1 antibody was generated as previously described [43 (link)]. Other antibodies were purchased as follows: anti-α-tubulin antibody was from Sigma-aldrich Co; anti-EGFR, anti-phospho-EGFR (Tyr1068), anti-phospho-HER3, anti-Met, anti-phospho-Met, anti-PDGFRβ, anti-phospho-PDGFRβ, anti- IGF-1Rβ, anti-phospho-IGF-1Rβ, anti-ERK1/2, anti-phospho-ERK1/2, anti-AKT, anti-phospho-AKT (Thr308 and Ser473), anti-mTOR, anti-phospho-mTOR (Ser2448 and Ser2481), anti-Raptor, anti-Rictor, anti-S6K, anti-phospho-S6K(Thr389), anti-phospho-S6, anti-PTEN, anti-phospho-PTEN, anti-IRS-1, anti-PDK1, anti-PARP, anti-phospho-PDK1, anti-TSC-1, anti-TSC-2, and anti-phospho-4EBP-1 antibodies were from Cell Signaling Technology (Beverly, MA); anti-HER2 and anti-HER3 antibodies were from Santa Cruz Biotechnology (Santa Cruz, CA); anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) antibody was from Trevigen Inc. (Gaithersburg, MD): anti-p27 antibody was from BD Biosciences (San Jose, CA): anti-cleaved PARP antibody was from Promega (Madison, WI): anti-phospho-HER2 antibody was from Millipore (Billerica, MA): anti-β-actin was from Abcam (Cambridge, UK).
Watari K., Nishitani A., Shibata T., Noda M., Kawahara A., Akiba J., Murakami Y., Yano H., Kuwano M, & Ono M. (2016). Phosphorylation of mTOR Ser2481 is a key target limiting the efficacy of rapalogs for treating hepatocellular carcinoma. Oncotarget, 7(30), 47403-47417.
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3

PDGFR Signaling Pathway Analysis

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PLX4032 was purchased from AdooQ BioScience (Irvine, CA, USA). Rabbit anti-phospho-PDGFR-α, anti-phospho-PDGFR-β, anti-PDGFR-α, anti-PDGFR-β, anti-glyceraldehyde 3-phosphate dehydrogenase antibodies, and secondary horseradish peroxidase-conjugated antibody were purchased from Cell Signaling Technology (Danvers, MA, USA). Tyrphostin AG1296 and other reagents and chemicals were purchased from Sigma-Aldrich (St Louis, MO, USA).
Li Y., Li Y., Liu Q, & Wang A. (2015). Tyrphostin AG1296, a platelet-derived growth factor receptor inhibitor, induces apoptosis, and reduces viability and migration of PLX4032-resistant melanoma cells. OncoTargets and therapy, 8, 1043-1051.
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4

Western Blotting Antibody Validation

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Western blotting was performed as described previously.34 (link) The anti-H3K27ac antibody (C15410174) was purchased from Diagenode (Denville, NJ, USA). The anti-PDGFC antibody (#ab93899) and anti-Ki67 (#ab156956) were purchased from Abcam. The anti-PIK3CA antibody (67071-1-Ig) was purchased from Proteintech (Rosement, IL, USA). The anti-β-actin (#4970); anti-histone H3 (#4499); anti-phospho-PDGFRα (#2992); anti-phospho-PDGFRβ (#2227); anti-AKT (#4691); anti-phospho-AKT (#4060); anti-PARP (#9532); anti-cleaved PARP (#5625); anti-caspase3 (#14220S); anti-cleaved caspase3 (#9664S); anti-caspase9 (#9508); anti-cleaved caspase9 (#7237), anti-rabbit immunoglobulin G (IgG), HRP-linked (#7071); and anti-mouse IgG, HRP-linked (#7076) antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA). The dilution ratios of the primary antibodies and the secondary antibodies were 1:1,000 and 1:5,000, respectively.
Shi Y.H., Xu Q.C., Zhu Y.Q., Liu Z.D., Zhao G.Y., Liu Q., Wang X.Y., Wang J.Q., Xu X., Su Q., Lai J.M., Huang C.S, & Yin X.Y. (2022). Imatinib facilitates gemcitabine sensitivity by targeting epigenetically activated PDGFC signaling in pancreatic cancer. Molecular Therapy, 31(2), 503-516.
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5

PDGF-BB Stimulation of NIH3T3 Cells

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NIH3T3 cells (1.5 × 105 cells/well in 6-well) were mock-treated or serum-starved for 18 h and then left untreated or stimulated for 10 min with 20 ng/mL PDGF-BB. Cell lysates preparation and immunoblotting analyses with anti-phospho-PDGFRβ (Tyr771, indicated as p-PDGFRβ) (Cell Signaling Technology Inc.) primary antibodies were performed as previously reported [42 (link)] by using anti-vinculin (N-19) (Santa Cruz Biotechnology, Santa Cruz, CA) as loading control. Blots shown are representative of at least three independent experiments.
Camorani S., Passariello M., Agnello L., Esposito S., Collina F., Cantile M., Di Bonito M., Ulasov I.V., Fedele M., Zannetti A., De Lorenzo C, & Cerchia L. (2020). Aptamer targeted therapy potentiates immune checkpoint blockade in triple-negative breast cancer. Journal of Experimental & Clinical Cancer Research : CR, 39, 180.
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