For MIRA, cGAS-mtDNA SIRF, DNA fibers, and immunofluorescence assays, slides were imaged using an Eclipse Ti-U inverted microscope with an Andor Zyla sCMOS camera or a Zeiss Airyscan LSM 800 microscope with a Hamamatsu C13440 camera. Images were analyzed using the Nikon NIS Elements or Zen2 software version 2.3. For ease of viewing, a black bar was placed over the scale bars of select images. Statistical analysis was performed using GraphPad Prism 7 software. Four to eight image fields were acquired for each condition, and the data from two to four biological replicates were compiled. For both MIRA and mitoSIRF assays, the cytoplasmic signals were counted irrespective of cell cycle status. P values were calculated using the Mann-Whitney test (GraphPad Prism 7 software).
Scmos camera
Manufactured by Zeiss
Sourced in Germany
The SCMOS camera is a scientific-grade camera that utilizes a scientific complementary metal-oxide-semiconductor (sCMOS) image sensor. The sCMOS sensor provides high-resolution imaging with low noise, high dynamic range, and rapid frame rates. The camera is designed for a variety of applications in scientific research and industrial imaging.
Automatically generated - may contain errors
2 protocols using scmos camera
1
Microscopy Imaging of DNA and Mitochondrial Signaling
2
Fluorescent Strain Microscopy Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For microscopy observation, the strains contain fluorescent fusion (s) were grown overnight on LB plates with gentamicin (50 μg/ml), then using sterile toothpicks to pick single colony into 10 μl PBS and 1 μl culture was spotted on a slide which was coated with 1% agarose. Phase contrast and fluorescence microscopy were performed by using Observer Z1 equipped with sCMOS camera (Zeiss, Germany).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!