Gestron 1500

Manufactured by Kyoritsu Seiyaku

Sourced in Japan

The Gestron 1500 is a laboratory equipment designed for precise and efficient liquid handling. It features a digital interface and advanced pipetting capabilities to ensure accurate and reproducible results.

Automatically generated - may contain errors

Lab products found in correlation

Planate, by MSD (3 mentions) Planate, by MSD animal health (3 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Four well dishes, by Thermo Fisher Scientific (1 mentions) Povidone iodine, by Meiji Seika Pharma (1 mentions)

4 protocols using gestron 1500

Porcine Oocyte In Vitro Maturation

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Ovaries were collected from prepubertal gilts at a local slaughterhouse and were maintained at 37ºC during transport to the laboratory. Cumulus-oocyte complexes (COCs) were obtained from
follicles 2–6 mm in diameter in TCM-199 medium supplemented with 10% (v/v) fetal bovine serum (FBS; Thermo Fisher Scientific, Kanagawa, Japan), 20 mM Hepes, 0.68 mM L-glutamine, 100 IU/ml
penicillin G potassium (Meiji Seika, Tokyo, Japan), and 0.1 mg/ml streptomycin sulfate (Meiji Seika). Approximately 50 COCs with uniform ooplasm and a cumulus cell mass were cultured
separately in four-well dishes (Thermo Fisher) for 20 h in 500 μl of maturation medium, composed of a modified North Carolina State University (NCSU)-37 (mNCSU-37) [27 (link)] solution containing 10% porcine follicular fluid, 0.6 mM cysteine, 0.05 mM β-mercaptoethanol, 1 mM dibutyryl cAMP (dbcAMP), 10 IU/ml pregnant mare serum gonadotropin
(Serotropin, Aska Animal Health, Tokyo, Japan), and 10 IU/ml human chorionic gonadotropin (hCG; Gestron 1500, Kyoritsu seiyaku, Tokyo, Japan). The COCs were subsequently cultured in
maturation medium without dbcAMP and hormones for 24 h. The maturation culture was performed at 39ºC in a humidified atmosphere containing 5% CO₂, 5% O₂, and 90%
N₂.

EMURA N., TAKAHASHI K., SAITO Y, & SAWAI K. (2019). The necessity of TEAD4 for early development and gene expression involved in differentiation in porcine embryos. The Journal of Reproduction and Development, 65(4), 361-368.

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Embryo Transfer in Recipient Gilts

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Recipient gilts, after estrous cycles were synchronized, were prepared for embryo transfer as described previously [46 (link)]. In brief, 0.2 mg of cloprostenol (Planate; MSD Animal Health, Tokyo, Japan) was administered by intramuscular (i.m.) injection to pregnant gilts 35 to 53 days after the day of insemination. Subsequently, a second intramuscular injection of 0.2 mg of cloprostenol and intramuscular injection of 1000 IU of eCG (PMSA for Animal, ZENOAQ, Fukushima, Japan) was administered to the gilts 24 h after the first injection of cloprostenol. At 72 h after the intramuscular injection of eCG, 1500 IU of hCG (Gestron 1500, Kyoritsu Seiyaku, Tokyo, Japan) was administered to the gilts. Approximately 72 h after the hCG i.m. injection, one- to two-cell stage embryos that were electroporated approximately 12 h before embryo transfer were transferred into the oviducts of a recipient gilt under anesthesia. The gilts were placed in the supine position, and the surgical area was disinfected with povidone–iodine (Meiji Seika Pharma, Tokyo, Japan). Approximately 100 zygotes were transferred to each oviduct, resulting in the transfer of 200 zygotes per gilt under sterile conditions.

Tanihara F., Hirata M., Nguyen N.T., Sawamoto O., Kikuchi T, & Otoi T. (2021). One-Step Generation of Multiple Gene-Edited Pigs by Electroporation of the CRISPR/Cas9 System into Zygotes to Reduce Xenoantigen Biosynthesis. International Journal of Molecular Sciences, 22(5), 2249.

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Synchronizing Estrus in Recipient Gilts for Embryo Transfer

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The preparation of recipient gilts for embryo transfer was performed as described previously (Onishi et al., 2000 (link)). Four to 7 weeks after mating, pregnant gilts were administered .2 mg of cloprostenol (Planate; MSD Animal Health, Tokyo, Japan) via intramuscular (i.m.) injection. After 24 h, the gilts were administered a second i.m. injection of .2 mg cloprostenol and 1000 IU eCG (PMSA for Animal, ZENOAQ, Fukushima, Japan). Then, 72 h after the eCG injection, recipient gilts were administered an i.m. injection of 1500 IU hCG (Gestron 1500, Kyoritsu Seiyaku) to induce the estrus. Subsequently, 100 embryos electroporated 12 h before embryo transfer were transferred into each oviduct of a recipient gilt 72 h after the hCG i.m. injection, resulting in the transfer of 200 embryos per gilt.

Tanihara F., Hirata M., Namula Z., Do L.T., Yoshimura N., Lin Q., Takebayashi K., Sakuma T., Yamamoto T, & Otoi T. (2023). Pigs with an INS point mutation derived from zygotes electroporated with CRISPR/Cas9 and ssODN. Frontiers in Cell and Developmental Biology, 11, 884340.

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Synchronization and Embryo Transfer in Gilts

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Recipient gilt, after synchronization of estrous cycles, was prepared for embryo transfer as described previously^{55 (link)}. In brief, 0.2 mg of cloprostenol (Planate; MSD Animal Health, Tokyo, Japan) was administered by intramuscular injection to pregnant gilt 4–7 weeks after mating. Subsequently, a second intramuscular injection of 0.2 mg of cloprostenol and 1000 IU of eCG (PMSG, ZENOAQ, Fukushima, Japan) was administered to the gilt 24 h after the first injection of cloprostenol. At 72 h after the intramuscular injection of eCG, 1500 IU of hCG (Gestron 1500, Kyoritsu Seiyaku, Tokyo, Japan) was administered to the gilt. Approximately 125 h after the hCG intramuscular injection, early blastocysts derived from embryos treated with the RNP transfection reagent were transferred into the uterus of a recipient gilt under anesthesia.

Hirata M., Wittayarat M., Namula Z., Le Q.A., Lin Q., Takebayashi K., Thongkittidilok C., Mito T., Tomonari S., Tanihara F, & Otoi T. (2021). Generation of mutant pigs by lipofection-mediated genome editing in embryos. Scientific Reports, 11, 23806.

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