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Horseradish peroxidase labelled goat anti rabbit igg

Manufactured by Beyotime
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Horseradish peroxidase‐labelled goat anti‐rabbit IgG is a secondary antibody conjugate. It is designed for use in various immunoassay techniques that require the detection of rabbit primary antibodies.

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Lab products found in correlation

Anti mpo, by Abcam (1 mentions) Anti α sma, by Abcam (1 mentions) Ab1416, by Abcam (1 mentions) Ab108599, by Abcam (1 mentions) Sc 150, by Santa Cruz Biotechnology (1 mentions) Ab92547, by Abcam (1 mentions) Ab9485, by Abcam (1 mentions) A0208, by Beyotime (1 mentions)

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Horseradish peroxidase (34 citations)

2 protocols using horseradish peroxidase labelled goat anti rabbit igg

1

Lung Tissue Histological Analysis

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After fixation of the lung tissue, the slices were embedded in paraffin. Sections of 3 μm were stained by haematoxylin and eosin (HE) to identify lung structure. For immunohistochemistry, primary antibodies included rabbit anti‐myeloperoxidase antibody (anti‐MPO; Abcam) and anti‐alpha smooth muscle actin antibody (anti‐α‐SMA; Abcam). Horseradish peroxidase‐labelled goat anti‐rabbit IgG (Beyotime Biotechnology) was used as a secondary antibody.
Zhang S., Jia X., Zhang Q., Zhang L., Yang J., Hu C., Shi J., Jiang X., Lu J, & Shen H. (2019). Neutrophil extracellular traps activate lung fibroblast to induce polymyositis‐related interstitial lung diseases via TLR9‐miR‐7‐Smad2 pathway. Journal of Cellular and Molecular Medicine, 24(2), 1658-1669.
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2

Quantitative Western Blot Analysis

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Western blot analysis was performed as early reported18 Proteins were incubated with antibodies against TFF3 (1:1000; ab108599; Abcam, Cambridge, MA, USA), C/EBPβ (sc‐150; Santa Cruz Biotechnology, Santa Cruz, CA, USA), Vimentin (1:1000, ab92547), E‐cadherin (1:50, ab1416) and GAPDH (1:2500; ab9485, Abcam), followed by incubation with the secondary antibody, horseradish peroxidase‐labelled goat anti‐rabbit IgG (1:2,000; A0208; Beyotime Institute of Biotechnology, Shanghai, China). Each sample was repeated in triplicate. ImageJ v1.48u software (National Institutes of Health, Bethesda, Maryland, USA) was utilized for quantification analyses.
Wu H., Gu J., Zhou D., Cheng W., Wang Y., Wang Q, & Wang X. (2020). LINC00160 mediated paclitaxel‐And doxorubicin‐resistance in breast cancer cells by regulating TFF3 via transcription factor C/EBPβ. Journal of Cellular and Molecular Medicine, 24(15), 8589-8602.
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