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Bn 2 protein analyzer

Manufactured by Siemens
Sourced in Germany

The BN II protein analyzer is a lab equipment product from Siemens. It is designed for the quantitative determination of proteins in a variety of sample types.

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3 protocols using bn 2 protein analyzer

1

Comprehensive Biomarker Assessment Protocol

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The UA1MG level was tested using the overnight clean midstream urine, which was sampled in the morning between 6: 00 a.m. and 11: 00 a.m., within 24 h of hospital admission, and sent to the Laboratory Medicine Department within 1 h for testing, as previously reported [9 (link)]. The nephelometry assay kit was used to test the UA1MG level with the BN II protein analyzer (Siemens, Germany), with a normal reference range provided by the manufacturer of 0.0 to 1.2 mg/dL. As previously described [7 (link)], patients had their peripheral blood draw sampled immediately on admission. Complete blood cell counts were measured using the fluorescent dyeing flowcytometry method with the XN3000 analyzer (Sysmex, Japan). The lipid panel, glycated hemoglobin A1c, and high-sensitivity C-reactive protein (hs-CRP) levels were collected on the morning of the second day after at least 8 h of fasting. The levels of cardiac enzymes involving troponin I (cTnI), creatine kinase myocardial band (CK-MB), and N-terminal pro-B-type natriuretic peptide (NT-proBNP) were collected on admission and every morning according to the hospital protocol. Blood biochemistry parameters and cardiac biomarkers were evaluated using test kits with AU5821 biochemistry analyzing equipment (Beckman Coulter Company), according to the manufacturer’s instructions.
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2

Blood Biomarker Profiling in Admissions

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The fasting venous blood of ulnar vein was collected
within 24 h of admission, which was centrifuged
at 1000×g for 20 min. The supernatant was
collected and stored at -20°C. WBC (white blood
cells), LY (lymphocyte count), NEUT (neutrophil
count), MONO (monocyte count) and ESR (erythrocyte
sedimentation rate) were detected using an automatic hematology analyzer. Serum level of CRP was
detected using the BNII protein analyzer (Siemens,
Germany).
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3

Renal Biopsy Biomarker Evaluation

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Gender, age, body weight and height, systolic blood pressure (SBP), diastolic blood pressure (DBP), creatinine (Scr), urine protein, albumin, body mass index (BMI), uric acid (UA); total cholesterol (TG), and total cholesterol [23 (link)] were recorded at the time of renal biopsy. Urine was collected in the morning after at least 9 h’ water prohibition. Urinary α1-MG was measured by rate nephelometry (BNII Protein Analyzer, Siemens, Germany). Urinary β2-MG was measured by chemiluminescent technique (IMMULITE 2000, Siemens, Germany). Urinary ORM and MAU were measured by Immunoturbidimetry (Dako, Denmark). Urinary RBP was measured by Immunoturbidimetry (Byron, Shanghai). Estimated GFR [24 (link)] was calculated using the CKD-EPI formula (2009) [25 (link)]. Patients were followed up for at least 6 months after recruitment.
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