Srebp1 mab

Liver samples were lysed in ice-cold RIPA buffer supplemented with 1 mM PMSF (Sigma, St. Louis, MO, USA) and then kept at 4 °C for 2 h. Supernatant was collected by centrifuge at 10,000× g for 15 min at 4 °C. For SDS–PAGE, samples were mixed with SDS sample buffer and incubated at 98 °C for 5 min. Western blot was performed with the following antibodies: β-actin mAb (#3700, Cell Signaling, Danvers, MA, USA), peroxisome proliferator activated receptor α (PPARα) pAb (ab8934, Abcam, Cambridge, UK), sterol regulatory element-binding transcription factor 1 (SREBP1) mAb (sc-365513, Santa Cruz Technology, Santa Cruz, CA, USA), and 3-hydroxy-3-methylglutaryl-CoA reductase (HMGCR) mAb (ab174830, Abcam).

The following monoclonal (mAb) and polyclonal (pAb) primary antibodies were used for western blot and IHC: NFYA pAb (1:500; Santa Cruz, no. sc-10779), NFYA mAb (1:500; Santa Cruz, no. sc-17753), E-Cadherin mAb (1:1000; BD, no. 610181), Vimentin mAb (1:1000; BD, no. 550513), SNAI 1 mAb (1:500; Santa Cruz, no. sc-28199), Flag mAb (1:1000; Sigma, no. F3165), FASN Rabbit mAb (1:1000 for western blot, 1:100 for IHC; CST, no. 3180), ACACA Rabbit mAb (1:1000 for western blot, 1:100 for IHC; CST, no. 3676), CPT1A Rabbit mAb (1:1000; Abcam, no. Ab234111), ACADVL mAb (1:1000; Santa Cruz, no. sc-376239), SREBP1 mAb (1:500; Santa Cruz, no. sc-13551), CD36 pAb (1:500; Santa Cruz, no. sc-9154), Keratin 14 pAb (1:500; BioLegend, no. 905304), Keratin 8 mAb (1:500; BioLegend, no. 904804), and α-Tubulin mAb (1:2000; Sigma, no. T5168).