Immunofluorescence was performed on 4-um-thick paraffin sections. After deparaffinization and rehydration, antigen retrieval with target retrieval solution (Dako, Carpinteria, CA, USA) was performed. The sections were blocked and incubated with diluted primary antibody (Dako) at 4 °C overnight. Mouse anti-TNNT2 (Abcam; 1:200, Cambridge, UK) antibody was used to stain myocardium. Next, the samples were incubated with anti-mouse immunoglobulin G Alexa Fluor 488 (Invitrogen; 1:500) secondary antibody for 60 min at room temperature and then stained with 4′,6-diamidino-2-phenylindole solution (VECTOR, Torrance, CA, USA) for nuclear staining. Images of the heart sections were visualized using an LSM 800 laser scanning microscope with Airyscan processing (Zeiss, Oberkochen, Germany).
Mouse anti tnnt2
Manufactured by Abcam
Sourced in United Kingdom
Mouse anti-TNNT2 is a monoclonal antibody targeted against the troponin T cardiac muscle protein. It is intended for use in research applications to detect and analyze the TNNT2 protein.
Automatically generated - may contain errors
2 protocols using mouse anti tnnt2
1
Immunofluorescence Imaging of Myocardial TNNT2
2
Immunofluorescence Microscopy of TNNT2 in Paraffin Sections
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Immunofluorescence was performed on 4-um-thick paraffin sections. After deparaffinization and rehydration, antigen retrieval with target retrieval solution (Dako) was performed in a decloaking chamber. The sections were blocked and incubated with diluted primary antibody (Dako) at 4°C overnight. The primary antibody used in this study was mouse anti-TNNT2 (Abcam; 1:200). After washing three times with 1% Tween 20 in PBS, the samples were incubated with secondary antibody for 60 min at room temperature in the dark. The secondary antibody used in this study was anti-mouse immunoglobulin G Alexa Fluor 594 (Invitrogen; 1:500). After washing again with 1% Tween 20 in PBS, the sections were stained with 4′,6-diamidino-2-phenylindole solution (VECTASHIELD) for nuclear staining and then mounted on slides. Imaging of the heart sections was performed with an LSM 800 laser scanning microscope with Airyscan processing (Zeiss).
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