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Quantikine human vegf immunoassay elisa kit

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The Quantikine Human VEGF Immunoassay ELISA kit is a quantitative sandwich enzyme immunoassay designed to measure human vascular endothelial growth factor (VEGF) levels in cell culture supernates, serum, and plasma.

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Quantikine® ELISA Human VEGF Immunoassay Quantikine Human Immunoassay ELISA kit Quantikine ELISA Immunoassay kit Quantikine Human VEGF Human VEGF Immunoassay Quantikine Human Immunoassay VEGF immunoassay kit Quantikine Immunoassay kits VEGF ELISA kit Quantikine ELISA kit

7 protocols using quantikine human vegf immunoassay elisa kit

1

Measuring VEGF Levels in Bevacizumab

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Secreted VEGF levels were determined in the serum of patient samples before and after treatment using the Quantikine ELISA human VEGF Immunoassay Kit (Minneapolis, MN, USA) as per the procedure described by the manufacturer (R&D Systems, Minneapolis, MN, USA). Vascular endothelial growth factor levels were compared before and after the treatment. The blood samples were collected at baseline, 48–72 h after the first infusion of bevacizumab, right before the third infusion of bevacizumab (pre-C3), and at the end of treatment.
Pili R., Liu G., Chintala S., Verheul H., Rehman S., Attwood K., Lodge M.A., Wahl R., Martin J.I., Miles K.M., Paesante S., Adelaiye R., Godoy A., King S., Zwiebel J, & Carducci M.A. (2017). Combination of the histone deacetylase inhibitor vorinostat with bevacizumab in patients with clear-cell renal cell carcinoma: a multicentre, single-arm phase I/II clinical trial. British Journal of Cancer, 116(7), 874-883.
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2

Quantifying VEGF Levels in Pulmonary Artery

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The Quantikine ELISA Human VEGF Immunoassay kit (R&D Systems Europe) was used to assay VEGF levels in protein extracts from pulmonary artery rings. VEGF in the samples was captured on microtiter plates precoated with a monoclonal anti-VEGF antibody and detected by an enzyme-linked polyclonal antibody specific for VEGF. The minimum detectable dose of VEGF was below 5.0 pg/mL.
Henno P., Grassin-Delyle S., Belle E., Brollo M., Naline E., Sage E., Devillier P, & Israël-Biet D. (2017). In smokers, Sonic hedgehog modulates pulmonary endothelial function through vascular endothelial growth factor. Respiratory Research, 18, 102.
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3

VEGF Immunoassay Using Quantikine ELISA

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Quantikine ELISA Human VEGF Immunoassay Kit (R&D Systems, MN, USA). Peroxidase-conjugated AffiniPure goat antihuman IgG, Fcγ fragment-specific (minimal cross-reaction to bovine, horse and mouse serum proteins; Jackson ImmunoResearch, PA, USA). Peroxidase-conjugated AffiniPure goat antihuman IgG, F(ab') 2 fragmentspecific (minimal cross-reaction to bovine, horse and mouse serum proteins; Jackson ImmunoResearch).
Sumner G., Georgaros C., Rafique A., DiCioccio T., Martin J., Papadopoulos N., Daly T, & Torri A. (2019). Anti-VEGF drug interference with VEGF quantitation in the R&D systems human quantikine VEGF ELISA kit. Bioanalysis, 11(5).
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4

Quantification of VEGF Protein Levels

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Total VEGF protein expression in conditioned medium was determined using the Quantikine Human VEGF Immunoassay ELISA kit (R&D Systems), according to the manufacturer’s protocol. Absorbance readings minus background were converted to pictograms using standard curves from the kit.
Tian S., Cao D., Zou H., Bai F., Wang Z., Pan S, & Feng M. (2015). Endothelial cell-targeted pVEGF165 polyplex plays a pivotal role in inhibiting intimal thickening after vascular injury. International Journal of Nanomedicine, 10, 5751-5768.
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5

VEGF Quantification by ELISA

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The VEGF ELISA was performed as per the instructions with the Quantikine Human VEGF Immunoassay ELISA Kit (R&D Systems, Minneapolis, MN, USA). Similar ELISA procedures were described by us previously57 (link).
Che P., Yang Y., Han X., Hu M., Sellers J.C., Londono-Joshi A.I., Cai G.Q., Buchsbaum D.J., Christein J.D., Tang Q., Chen D., Li Q., Grizzle W.E., Lu Y.Y, & Ding Q. (2015). S100A4 promotes pancreatic cancer progression through a dual signaling pathway mediated by Src and focal adhesion kinase. Scientific Reports, 5, 8453.
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6

Quantifying VEGF Secretion

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The cell culture supernatants with or without proinflammatory cytokines and NF-κB inhibitor were collected for determining concentration by using the human ELISA development kit according to the manufacturer’s instruction (Quantikine Human VEGF Immunoassay ELISA Kit; R&D Systems, Minneapolis, MN, USA).
Buyandelger U., Walker D.G., Yanagisawa D., Morimura T, & Tooyama I. (2020). Effects of FTMT Expression by Retinal Pigment Epithelial Cells on Features of Angiogenesis. International Journal of Molecular Sciences, 21(10), 3635.
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7

VEGF Quantification in Heart Tissue

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VEGF expression was measured using the Quantikine Human VEGF Immunoassay ELISA kit (R&D Systems, Inc. Minneapolis, MN 55413). This is a solid phase ELISA designed to measure VEGF165 levels in the supernatant of the heart. It contains Sf 21-expressed recombinant human VEGF165 and antibodies raised against the recombinant protein. The kit includes plates pre-coated with a monoclonal antibody specific for VEGF. Tissue samples were homogenized and standards and samples were pipetted into the wells. Any VEGF present was bound by the immobilized antibody. After washing away any unbound substances, an enzyme-linked polyclonal antibody specific for VEGF was added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution was added to the wells, and color developed in proportion to the amount of VEGF bound in the initial step. The color development was stopped and the intensity of the color was measured.
Hargrave B., Strange R J.r., Navare S., Stratton M., Burcus N., Murray L., Lundberg C., Bulysheva A., Li F, & Heller R. (2014). Gene Electro Transfer of Plasmid Encoding Vascular Endothelial Growth Factor for Enhanced Expression and Perfusion in the Ischemic Swine Heart. PLoS ONE, 9(12), e115235.
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