The immunochromatographic strip consisted of five sections, which were sourced from Shanghai Goldbio Technology Co., Ltd., unless stated otherwise. The section consisted of the following components: A sample pad fiberglass membrane (SB06), a conjugate pad polyester fiber membrane (VL78), an analyzing nitrocellulose membrane (Merck Millipore), an absorbent pad (CH27) and a plastic back plate. The conjugate pad was pretreated with the purified colloidal gold-McAb KIM-1 conjugates, and then dried in an incubator at 37°C for 30 min. A control (C) line and test (T) line were marked on the analyzing membrane with goat anti-mouse IgG (2 mg/ml; BAF007, R&D Systems, Inc.) and goat anti-human PcAb KIM-1 (200 µg/ml, dilution, 1:200, AF1750, R&D Systems, Inc.), respectively. The sample pad, pretreated conjugate pad, analyzing membrane and absorbent pad were pasted on a plastic back plate. The detection limit of the colloidal gold-based immunochromatographic strip was determined by detecting normal urine specimens supplemented with KIM-1 at the final concentrations of 0, 50 and 100 ng/ml, and 1 and 10 µg/ml, respectively.
Baf007
Manufactured by R&D Systems
BAF007 is a laboratory instrument designed for the analysis of biological samples. It is capable of performing various analytical procedures, including protein quantification, enzyme activity assays, and immunoassays. The device utilizes established techniques to generate accurate and reliable results for researchers and scientists.
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Lab products found in correlation
2 protocols using baf007
1
Colloidal Gold-Based Immunochromatographic Assay for KIM-1 Detection
2
Western Blot Analysis of HNF4G Protein
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48 h after transfection, the cells were lysed in cell lysis buffer. The total protein concentration of the lysates was determined with the BCA Protein Assay kit (Pierce) according the manual. Protein sample (30 μg) was separated on 10% SDS-PAGE and then transferred onto nitrocellulose membrane. Membranes were blocked with 5% nonfat dried milk for 1 h and then incubated overnight with primary antibody. After three times washing with 1x TBS buffer containing 0.1% Tween 20, the membrane was further incubated with corresponding secondary antibody for 1 h. Signal intensities were detected using the Odyssey Infrared Imaging System (Li-Cor Biosciences). The primary antibodies and the working concentrations were mouse anti-HNF4G (R&D system, Clone N3224, 1 μg/mL) and mouse anti-GAPDH (R&D system, AF5718, 1 μg/mL). The second antibody used was Horseradish peroxidase-conjugated anti-mouse IgG (R&D system, BAF007, 1 : 5000).
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