Anti-SM22 (1:100, ab10135, Abcam, UK), Goat isotope control IgG (5 μg/mL, ab18433 Abcam), Rabbit anti-ETB receptor (1:100, ab117529, Abcam), Rabbit isotope control IgG (10 μg/mL, ab18433, Abcam), Allophycocyanin (APC)-conjugated donkey anti-rabbit IgG (1:100, Jackson ImmunoResearch), Alexa 488-conjugated donkey anti-goat IgG (1:100, Jackson ImmunoResearch), Fixable Viability Dye eFluor 780 (eBioscience), Liberase TM Research Grade (Roche), German coverslip with Poly-L-lysine coating on both sides cat no H-12-pII Neuvitro, USA, Vectashield Antifade Mounting Medium (Vector Laboratories, USA), BSA (Bovine Serum Albumin, Sigma Aldrich), FBS (Fetal Bovine Serum, Sigma Aldrich), Triton X-100 (Sigma Aldrich).
Radziwon-Balicka A., Degn M., Johansson S.E., Warfvinge K, & Edvinsson L. (2017). A novel multicolor flow-cytometry application for quantitative detection of receptors on vascular smooth muscle cells. PLoS ONE, 12(10), e0186504.
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