After euthanasia, the subcutaneous implants were fixed with 10% formalin. Following fixation, the implants were washed and placed into a processor that dehydrated the samples in 70% alcohol, followed by 95%, 100%, and xylene. The samples were then embedded in paraffin and cut into slices of 5 microns using a microtome (Accu-Cut SRM 200 Rotary Microtomoe, Sakura Finetek USA, CA). Slides were stained with hematoxylin and eosin (H&E) and Goldner’s trichrome (Sigma-Aldrich). Images were obtained with Lionheart LX (Biotek Instruments Inc, Winooski, VT) at 4× and captured using Gen 5 software.
Accu cut srm 200 rotary microtomoe
Manufactured by Sakura Finetek
Sourced in United States
The Accu-Cut SRM 200 Rotary Microtome is a laboratory equipment used for preparing thin sections of biological samples for microscopic analysis. It is designed to provide precise and consistent tissue sectioning with its rotary cutting mechanism.
Automatically generated - may contain errors
3 protocols using accu cut srm 200 rotary microtomoe
1
Histological Evaluation of Subcutaneous Implants
2
Histological Analysis of Subcutaneous Implants
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After euthanasia, at 4 weeks post-surgery, the subcutaneous implants were fixed with 10% formalin. Following fixation, the implants were washed and placed into a processor which dehydrated the samples sequentially in 70%, 95% and 100% alcohol, followed by xylene. The samples were then embedded in paraffin and cut into slices of 5 microns using a microtome (Accu-Cut SRM 200 Rotary Microtomoe, Sakura Finetek USA, Torrance, CA, USA). Slides were stained with Hematoxylin and Eosin (H&E) and Goldner’s trichrome (Sigma-Aldrich). Images were obtained with Lionheart LX (Biotek Instruments Inc., Winooski, VT, USA) at 4× and captured using Gen 5 software (Biotek Instruments Inc., Winooski, VT, USA)).
3
Histological Analysis of Rat Femurs
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Rat femurs were fixed in 10% NBF and decalcified with Cal-Ex® II (Fisher Chemical CS5114D Cal-Ex® II Fixative/Decalcifier). Following decalcification and fixation, samples were dehydrated in increasing concentrations of alcohol (70%, 95%, 100%), followed by xylene before embedding in paraffin and cut into 5 micron slices (Accu-Cut SRM 200 Rotary Microtomoe, Sakura Finetek USA, Torrance, CA, USA). Slides were stained with Hematoxylin and Eosin, Goldner’s Trichrome, and Safranin-O/Fast-green. Images were obtained with Aperio VERSA200 (Leica Biosystems, Inc., Buffalo Grove, IL, USA) with a 40X HC PL APO objective with .95 numerical aperture and captured with Aperio ImageScope v12.4.3 (Leica Biosystems, Inc., Buffalo Grove, IL, USA).
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