Rwpe 1

RWPE-1, 22RV1, PC-3, and DU145 cell lines were used in subsequent validation experiments. All these cell lines were obtained from Procell Life Science and Technology Co., Ltd. (Wuhan, China). In brief, RPMI-1640 medium (Biological Industries, Beit Haemek, Israel) with 10% fetal bovine serum (FBS) was used to culture RWPE-1 cell line and 22RV1 cell line. Comparatively, the DU145 and PC-3 cells were cultured in corresponding cell specific medium. All these cell specific media were also provided by Procell Life Science and Technology Co., Ltd (Wuhan, China). Most importantly, the cells were strictly incubated at 37 degrees and 5% CO₂.

Human PCa cell line DU145 (Procell CL-0075) and normal prostatic epithelial cell line RWPE-1(Procell CL-0200) were kindly provided by Procell Life ScienceandTechnology Co., Ltd. The DU145 cell line was cultured in MEM medium (Biological Industries, Beit HaEmek, Israel), supplemented with 10% fetal bovine serum in a standard humidified incubator at 37°C in a 5% CO2 atmosphere. The RWPE-1 cell line was cultured in RPMI-1640 medium (Biological Industries, Beit HaEmek, Israel) supplemented with 10% fetal bovine serum at 37°C in a humidified atmosphere containing 5% CO2.