Plastic tubes were used to collect blood samples, from which the serum was obtained through centrifugation at 3,000 g for 10 minutes at a temperature of 4 ℃. The obtained serum was conserved at −80 ℃ for subsequent analysis. To minimize the impact of the circadian rhythm of the HPA axis on the results, all samples within each group were gathered in the same time window (from 10:00 AM to 12:00 AM). Concentrations of interleukin (IL)-1β, IL-6, IL-8, and tumor necrosis factor alpha (TNF-α) inside the serum samples were measured via commercial ELISA kits (product codes: MM-0047R1, MM-0190R1, MM-0175R2, MM-0180R2) by Jiangsu Meimian Industrial Co., Ltd. according to the producer’s instructions. Plates were read by 1 ELISA reader at a 450-nm wavelength. The sensitivity of the kit for IL-1β was 0.1 pg/mL, while that for the IL-6, IL-8, and TNF-α kits was 1.0 pg/mL.
Mm 0180r2
Manufactured by Jiangsu Meimian
Sourced in China
The MM-0180R2 is a laboratory equipment product. It is a device designed for use in a laboratory setting, but without further details, a more specific description cannot be provided while maintaining an unbiased and factual approach.
Automatically generated - may contain errors
2 protocols using mm 0180r2
1
Quantifying Inflammatory Markers in Serum
2
Inflammatory Cytokine Quantification by ELISA
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The inflammatory cytokines were measured by enzyme-linked immunosorbent assays (ELISA) according to the instructions. The interleukin 6 (IL-6) (MM-0190R2, MEIMIAN, China), IL-10 (PI525, Beyotime, China), IL-1β (MM-0099R2, MEIMIAN, China), and TNF-α (MM-0180R2, MEIMIAN, China) were used for evaluating the level of inflammation in serum. The OD was measured at 450 nm by a microplate reader (CMaxPlus, Molecular Devices, USA). Their concentration was directly proportional to the OD (450 nm) value, which was calculated by plotting a standard curve.
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