Following gene transfection or drug treatments, cell lysates were prepared and quantified before performing further experiments. Western blotting analysis was carried out as previously described (Wang et al, 2013a (link)). The following antibodies were used: anti-ROC1 (Abcam, Cambridge, MA, USA); anti-Phospho-4E-BP1 (Thr37/46) and anti-E-cadherin (Cell Signaling); anti-non-4E-BP1 and anti-glyceraldehyde-3-phosphate dehydrogenase (anti-GAPDH; Epitomics, Hangzhou, China); anti-vimentin and anti-N-cadherin (Santa Cruz); and anti-DEPTOR (Sigma).
Anti roc1
Manufactured by Abcam
Sourced in United States
Anti-ROC1 is a protein-specific antibody that recognizes the ROC1 (RBX1) protein. ROC1 is a component of the SCF ubiquitin ligase complex, which plays a role in the ubiquitination and degradation of cellular proteins. This antibody can be used for the detection and analysis of ROC1 in various research applications.
Automatically generated - may contain errors
Lab products found in correlation
Anti ha, by Abcam (5 mentions)
Anti gli2, by Santa Cruz Biotechnology (5 mentions)
Anti cdc25c, by Abcam (5 mentions)
Anti sufu, by Abcam (5 mentions)
Anti gli1, by Abcam (5 mentions)
Anti cyclin d1, by Abcam (5 mentions)
Anti gapdh, by Abcam (5 mentions)
Anti ddb1, by Santa Cruz Biotechnology (2 mentions)
Ecl kit, by Thermo Fisher Scientific (2 mentions)
Anti e cadherin, by Cell Signaling Technology (1 mentions)
Protease inhibitor mixture cocktail, by Roche (1 mentions)
Short hairpin rnas shrnas, by GenePharma (1 mentions)
Anti suz12, by Cell Signaling Technology (1 mentions)
Dynabeads protein g, by Thermo Fisher Scientific (1 mentions)
Anti dnmt3b, by Abcam (1 mentions)
Anti lsd1, by Abcam (1 mentions)
Anti sin3a, by Santa Cruz Biotechnology (1 mentions)
Anti α catenin, by BD (1 mentions)
Anti wdr77, by Fortis Life Sciences (1 mentions)
Anti eed, by Merck Group (1 mentions)
9 protocols using anti roc1
1
Western Blot Analysis of Cell Signaling
2
Comprehensive Chromatin Remodeling Antibody Panel
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The sources of the antibodies were anti-FLAG, anti-HDAC1 anti-HDAC2, anti-RbAp46/48, anti-Fibronectin, anti-Vimentin and anti-β-actin (Sigma–Aldrich); anti-DDB1, anti-MTA1, anti-SIN3A, anti-SAP180 and anti-SAP30 (Santa Cruz Biotechnology); anti-PRMT5, anti-ROC1, anti-LSD1, anti-DNMT3B, anti-HDAC5 and anti-MTA2 (Abcam); anti-EED, anti-MBD2/3 and anti-MTA3 (Millipore); anti-SUZ12 (Cell Signaling Technology); anti-WDR77 (also known as MEP50, Bethyl); anti-MTA2, anti-E-cadherin, anti-α-catenin, anti-γ-catenin, anti-N-cadherin and anti-EZH2 (BD Bioscience); anti-ING4 (Genetex), anti-SETD2 (Proteintech). Dynabeads Protein G was obtained from Invitrogen by Thermo Fisher Scientific, and protease inhibitor mixture cocktail was from Roche Applied Science. Glutathione-Sepharose 4B beads were from GE Healthcare Bio-Sciences. Short hairpin RNAs (shRNAs) were obtained from GenePharma Co Ltd (Shanghai, China).
3
Investigating Protein Interactions via WB and Co-IP
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After the cells were subjected gene transfection or drug treatments, cell lysates were prepared and quantified according to a previous study [14 (link)]. The western blot was carried out as described previously [13 (link)], while the co-immunoprecipitation kit (Cat. #26419) from Thermo Scientific (Waltham, MA, USA) was used according to the manufacturer’s instructions with the following antibodies: anti-ROC1 (Abcam, Cambridge, MA, USA); anti-cyclin D1, anti-Cdc25c, anti-SUFU, anti-Gli1, anti-GAPDH, and anti-HA (Abcam, Hangzhou, China); and anti-Gli2 (Santa Cruz Biotechnology, Santa Cruz, CA, USA).
4
Western Blot Analysis of Protein Expression in Mouse Tissue and Organoids
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Proteins were extracted from mouse tissue and organoids. 25 micrograms of protein were immunoblotted by standard protocols. The primary antibodies included as the following: anti-CUL4B (Sigma), anti-Lgr5 (BBI Life Sciences, Shanghai, China), anti-Histone (GeneTex), anti-α-Tubulin (Proteintech, Wuhan, China), anti-Cytokeratin 20 (GeneTex), anti-GAPDH, anti-pGSK3β(Ser9), anti-p-β-catenin (S33/37/T41), anti-Non-p-β-catenin, anti-Ub (all antibodies from Cell signaling), anti-IRGM1(GeneTex, Cell signaling), anti-PTGES3 (Proteintech), anti-SLC5A1, anti-ABCG2, anti-STX18 (ORIGENE, Rockville, USA), anti-EPCAM (Abways, Shanghai, China), anti-ACTIN, anti-DDB1, anti-β-catenin (Santa Cruz), anti-HA (Rockland) anti-ROC1 and anti-WDR77, anti-WDR1(Abcam). The secondary antibodies included anti-rabbit and anti-mouse horseradish peroxidase (HRP) (Jackson ImmunoResearch, West Grove, PA, USA; 1:10000; 1:5000). The detection reagent Cheminoluminiscent Substrate was provided from the ECL kit (Thermo, USA). The band signals from Western blot results were analyzed by Volume Analysis of Quantity One with volume background subtraction (Bio-Rad, USA).
5
Multifunctional Nanoparticles for Cancer Therapy
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Polyethylenimine (PEI, MW 10000), dopamine hydrochloride and folic acid (FA) were purchased from Macklin. N-(3-Dimethylaminopropyl)-Nʹ-ethylcarbodiimide hydrochloride (EDC), N-hydroxysuccinimide (NHS) and Hoechst 33258 were purchased from Sigma. The other chemical reagents used in this study were all analytically pure. Cy3-siRNA was purchased from Shanghai GenePharma Co., Ltd. LysoTracker® Green was purchased from Beyotime Biotechnology. BCA protein assay kits were purchased from Beyotime Biotechnology. Anti-ROC1, anti-Cullin1 (anti-Cul1), anti-Cullin5 (anti-Cul5), anti-ATF4, anti-P-H2AX, anti-cleaved caspase 3 and anti-Ki-67 antibodies were purchased from Abcam. CCK-8 (Cell Counting Kit-8) was purchased from MedChemExpress. An Annexin V FITC apototic kit was purchased from BD Biosciences.
6
Protein Interactome Analysis by Co-IP
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After the cells were subjected gene transfection or drug treatments, cell lysates were prepared and quanti ed according to a previous study [14] . The western blot was carried out as described previously [13] , while the co-immunoprecipitation kit (Cat. #26419) from Thermo Scienti c (Waltham, MA, USA) was used according to the manufacturer's instructions with the following antibodies: anti-ROC1 (Abcam, Cambridge, MA, USA); anti-cyclin D1, anti-Cdc25c, anti-SUFU, anti-Gli1, anti-GAPDH, and anti-HA (Abcam, Hangzhou, China); and anti-Gli2 (Santa Cruz Biotechnology, Santa Cruz, CA, USA).
7
Western Blot and Co-Immunoprecipitation Analysis
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After cells were subjected gene transfection or drug treatments, cell lysates were prepared and quanti ed according to a previous study [14] . The Western blot was carried out as described previously [13] , while the co-immunoprecipitation (Co-IP) Kit (Cat. #26419) from Thermo Scienti c (Waltham, MA, USA) was used according to the manufacturer's instructions with the following antibodies, i.e., an anti-ROC1 (Abcam, Cambridge, MA, USA), anti-cyclinD1, anti-Cdc25c, anti-SUFU, anti-Gli1, anti-GAPDH, anti-HA (all from Abcam, Hangzhou, China), or anti-Gli2 (Santa Cruz Biotechnology, Santa Cruz, CA, USA) antibody.
8
Protein Interactome Analysis by Co-IP
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After the cells were subjected gene transfection or drug treatments, cell lysates were prepared and quanti ed according to a previous study [14] . The western blot was carried out as described previously [13] , while the co-immunoprecipitation kit (Cat. #26419) from Thermo Scienti c (Waltham, MA, USA) was used according to the manufacturer's instructions with the following antibodies: anti-ROC1 (Abcam, Cambridge, MA, USA); anti-cyclin D1, anti-Cdc25c, anti-SUFU, anti-Gli1, anti-GAPDH, and anti-HA (Abcam, Hangzhou, China); and anti-Gli2 (Santa Cruz Biotechnology, Santa Cruz, CA, USA).
9
Western Blot and Co-Immunoprecipitation Analysis
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After cells were subjected gene transfection or drug treatments, cell lysates were prepared and quanti ed according to a previous study [14] . The Western blot was carried out as described previously [13] , while the co-immunoprecipitation (Co-IP) Kit (Cat. #26419) from Thermo Scienti c (Waltham, MA, USA) was used according to the manufacturer's instructions with the following antibodies, i.e., an anti-ROC1 (Abcam, Cambridge, MA, USA), anti-cyclinD1, anti-Cdc25c, anti-SUFU, anti-Gli1, anti-GAPDH, anti-HA (all from Abcam, Hangzhou, China), or anti-Gli2 (Santa Cruz Biotechnology, Santa Cruz, CA, USA) antibody.
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