Sst ires cre knock in homozygous mice

All experiments were performed with 3- to 5 month-old male and female mice littermates, between 9 am and 3 pm. All non-stressed control mice were group housed and maintained under standard conditions (12/12-hour light/dark cycle, lights on at 07:00 h, 22±1°C, with food and water ad libitum). All mice were on the C57BL/6J background. The Sst^KO mouse line was obtained from the Jackson Laboratory (stock no. 008117).^{17 (link)}Sst^KO, Sst^HZ, Sst^WT littermates were generated by crossing Sst^HZ mice. Genotyping was done by Polymerase Chain Reaction (PCR) analysis of DNA isolated from tail cuts. To generate mice with green fluorescent protein (GFP) expression in SST cells, the mouse line carrying a somatostatin promoter-driven Cre recombinase expression (SST-ires-cre knock-in homozygous mice; Jackson Laboratory; stock no. 013044) was crossed with a Rosa^GFP-floxed reporter mouse line carrying a loxP-flanked STOP cassette (Ai6 Rosa26^{-loxP-STOP-loxP-ZsGreen}; Jackson Laboratory; stock no.008242), as described previously.^{40 (link)}