Salidroside (SAL) was purchased from the National Institute for the Control of Pharmaceutical and Biological Products (Beijing, China). Resveratrol (3,5,4′-trihydroxystilbene), H2DCFDA (2′,7′-dichlorodihydrofluorecein diacetate), NAO (nonyl acridine orange), and JC-1 (5,5′,6,6′-tetrachloro-1,1′,3,3′-tetraethyl benzimidazole carbocyanine iodide) were purchased from Sigma-Aldrich. Selisistat (EX-527) was from MedChemExpress (MCE). Dulbecco's modified Eagle's medium (DMEM), fetal bovine serum (FBS), and trypsin were obtained from Invitrogen, USA. Primary anti-PGC-1α antibody was obtained from Novus Biologicals, LLC, USA. Antibodies for SIRT1, Rb, p21Waf1, and p16INK4a were from Cell Signaling Technology, Inc. Anti-NFR-1 and anti-TFAM antibodies were Abcam products. Anti-p53 and anti-β-actin primary antibodies were purchased from Santa Cruz Biotechnology, Inc., USA.
Selisistat ex 527
Manufactured by MedChemExpress
Selisistat (EX-527) is a selective and potent inhibitor of the enzyme sirtuin 1 (SIRT1). It is a small molecule compound that has been widely used in research studies to modulate SIRT1 activity.
Automatically generated - may contain errors
Lab products found in correlation
Trypsin, by Thermo Fisher Scientific (1 mentions)
P16ink4a, by Cell Signaling Technology (1 mentions)
H2dcfda, by Merck Group (1 mentions)
Resveratrol, by Merck Group (1 mentions)
Glx351322, by MedChemExpress (1 mentions)
Ml385, by MedChemExpress (1 mentions)
Nigericin, by InvivoGen (1 mentions)
Anti asc ag 25b 0006, by Adipogen (1 mentions)
Mctr1, by Cayman Chemical (1 mentions)
Sdc 1, by R&D Systems (1 mentions)
Paraformaldehyde (pfa), by Merck Group (1 mentions)
Il 1β, by R&D Systems (1 mentions)
Tnf α, by R&D Systems (1 mentions)
Interleukin 6 (il 6), by R&D Systems (1 mentions)
4 protocols using selisistat ex 527
1
Salidroside Modulates Cellular Senescence
2
Inhibition of NOX4, Sirt1, and Nrf2
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The NOX4 inhibitor GLX351322, Sirt1-specific inhibitor selisistat (EX527), nuclear factor erythroid-2-related factor 2 (Nrf2)-specific inhibitor ML385 and ET-1 were purchased from MedChemExpress. The dosages of these inhibitors were determined based on the IC50 concentration (GLX351322, 5 µM; EX527, 123 nM; and ML385, 1.9 µM) and the results of the pre-tests.
3
Melatonin Neuroprotection in MPTP Model
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Melatonin (M5250), MPTP-HCL (M0896), 1-methyl-4-phenylpyridinium (MPP+, D048), adenosine 5-triphosphate disodium hydrate (ATP, A6419) were obtained from Sigma‒Aldrich (St Louis, MO), and nigericin was purchased from InvivoGen (San Diego, CA). Selisistat (EX-527) was purchased from MedChemExpress (Monmouth Junction, NJ). Anti-NLRP3 (AG-20B-0014), anti-caspase1 (AG-20B-0042), and anti-ASC (AG-25B-0006) antibodies were purchased from AdipoGen Life Sciences (San Diego, CA). Anti-IBA-1 (ab5076) antibody and anti-NF-κB p65 (ab16502) antibody were purchased from Abcam (Cambridge, MA). Anti-tyrosine hydroxylase (TH) (AB152) was purchased from Merck Millipore (Billerica, MA). Anti-SIRT1 (No.8469) antibody and anti-phospho-NF-κB p65 (No.3033) were purchased from Cell Signaling Technology (Danvers, MA). Anti-IL18 (No. A1115) antibody was purchased from ABclonal (Woburn, MA). Alexa fluor-488 conjugated anti-rabbit and Alexa fluor-546 conjugated anti-goat antibodies were purchased from Invitrogen (Carlsbad, CA). The mouse IL-1β ELISA kit (EK0394) was purchased from Boster Bio (Pleasanton, CA). The 2ʹ,7ʹ-dichlorodihydrofluorescein diacetate (DCFH-DA) fluorescent probe was purchased from NJJCBIO (Nanjing, China).
4
Inflammatory Response Regulation Protocol
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MCTR1 was obtained from Cayman Chemical (Ann Arbor, MI). Lipopolysaccharide (LPS), dimethyl sulfoxide (DMSO) and paraformaldehyde were purchased from Sigma-Aldrich (St. Louis, MO). The kits for measuring the plasma concentrations of TNF-α, IL-6, IL-1β, HS, and SDC-1 were obtained from R&D Systems (Minneapolis, MN). A rabbit polyclonal antibody against p-p65 was purchased from Cell Signaling Technology (Beverly, MA). A mouse anti-heparan sulfate proteoglycan 2/perlecan antibody, rabbit polyclonal antibodies against HPA and NF-κB/p65, mouse polyclonal antibodies against SIRT1 and HSPG2 and a donkey antibody against mouse IgG (H+L: Alexa Fluor® 594) were purchased from Abcam (Cambridge, MA). Mouse polyclonal antibodies against β-actin were purchased from ZSGB-BIO (Shanghai, China).
Peroxidase-conjugated goat antibodies against rabbit or mouse IgG (H+L) were purchased from BOYUN (Shanghai, China) . Selisistat (EX527), an inhibitor of SIRT1 enzymatic activity, was purchased from MedChem Express (Shanghai, China). BOC-2 (ALX inhibitor) was obtained from Biomol-Enzo Life Sciences (Farmingdale, NY).
Peroxidase-conjugated goat antibodies against rabbit or mouse IgG (H+L) were purchased from BOYUN (Shanghai, China) . Selisistat (EX527), an inhibitor of SIRT1 enzymatic activity, was purchased from MedChem Express (Shanghai, China). BOC-2 (ALX inhibitor) was obtained from Biomol-Enzo Life Sciences (Farmingdale, NY).
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