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Goat anti human fc hrp

Manufactured by Abcam
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Goat anti-human Fc-HRP is a secondary antibody conjugated with horseradish peroxidase (HRP). It binds to the Fc region of human antibodies, allowing for the detection of human immunoglobulins in various immunoassays.

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Lab products found in correlation

3 3 5 5 tetramethylbenzidine tmb reagent, by Merck Group (1 mentions)

Close spelling variants of this product

Goat anti-human IgG Fc-HRP antibody Goat anti-human IgG Fc-HRP

2 protocols using goat anti human fc hrp

1

Analysis of Immune Checkpoint Proteins

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Western blots were analyzed with goat anti-human Fc-HRP, biotinylated anti-human PD-1 Fab and anti-human LAG3 Fab. The dilution factor of goat anti-human Fc-HRP is 1:10,000 (Abcam). The membranes were cut prior to hybridisation with antibodies.
Zhao C., Zhang W., Gong G., Xie L., Wang M.W, & Hu Y. (2021). A new approach to produce IgG4-like bispecific antibodies. Scientific Reports, 11, 18630.
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2

FcγR Binding Assay Protocol

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The FcγRs RI, RIIA, RIIB, RIIIA (158V), and RIIIA (158F) were produced by MedImmune and coated on high binding CostarTM 96-well plates (Fisher Scientific) at a concentration of 2 μg/ml in PBS (pH 7.4) at 4 °C overnight. Immune complexes were prepared at a final antibody concentration of 15 μg/ml. 3-fold dilutions were made in PBS (pH 7.4), added to wells, and incubated for 2 h at 25 °C. Bound mAb or BiSAb was detected with goat anti-human Fc-HRP (Abcam, San Francisco, CA) using 3,3′,5,5′-tetramethylbenzidine (TMB) reagent (Sigma). Data were analyzed using GraphPad Prism.
Kasturirangan S., Rainey G.J., Xu L., Wang X., Portnoff A., Chen T., Fazenbaker C., Zhong H., Bee J., Zeng Z., Jenne C., Wu H, & Gao C. (2017). Targeted Fcγ Receptor (FcγR)-mediated Clearance by a Biparatopic Bispecific Antibody. The Journal of Biological Chemistry, 292(10), 4361-4370.
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