Agilent 5975 gc ms system

CBL extract was dissolved in n-hexane and the mixture was centrifuged thrice at 12,000 rpm for 15 min for GC-MS analyses. Supernatant was collected and used for GC–MS analysis. Agilent 5975 GCMS system (Agilent Technologies, USA) attached with HP-5 ms Capillary Column (30 m × 0.25 mm i.d. × 0.25 μm film thickness) and equipped with inert MSD triple axis mass detector condition edation trap 200°C, transfer line 280°C, electronenergy70eV (vacuum pressure-2.21e0.5 Torr) was used to identify the bio active compounds present in CBL extract. Helium was used as a carrier gas at a flow rate of 1 ml/min and 2 ml sample was injected in a split less mode. The column temperature was set at 60°C for 1 min followed by 5°C/min up to 250°C and the essential compounds in CBL were identified by the retention times and mass fragmentation patterns using Agilent Chem Station integrator and the database of National Institute of Standard and Technology (NIST) with a MS library version2011.