After the treatments, cell culture supernatants were cleared of cell debris by centrifugation at 1000 rpm for 20 min, and the concentrations of LDH (SEB864Ra, Cloud-Clone Corp, CCC, USA), CK-MB (SEA479Ra, Cloud-Clone Corp, CCC, USA), IL-1β (SEA563Ra, Cloud-Clone Corp, CCC, USA), and IL-18 (SEA064Ra, Cloud-Clone Corp, CCC, USA) were measured using ELISA kits according to the manufacturer’s instructions. The absorbance of each well at 450 nm was measured using a microplate reader.
Il 18
Manufactured by Cloud-Clone
Sourced in United States
The IL-18 is a laboratory equipment used for the detection and quantification of the cytokine Interleukin-18 (IL-18) in various biological samples. It functions as an analytical tool to measure IL-18 levels, which is important for research related to inflammatory processes and immune system responses.
Automatically generated - may contain errors
Lab products found in correlation
Il 1β, by Cloud-Clone (2 mentions)
Seb864ra, by Cloud-Clone (1 mentions)
Sea479ra, by Cloud-Clone (1 mentions)
Sea563ra, by Cloud-Clone (1 mentions)
Ifn β, by MyBioSource (1 mentions)
Tgf β, by MyBioSource (1 mentions)
Ifn α, by MyBioSource (1 mentions)
Il 10, by MyBioSource (1 mentions)
Tnf α, by MyBioSource (1 mentions)
Interleukin 2 (il 2), by Cloud-Clone (1 mentions)
Il 1β, by Thermo Fisher Scientific (1 mentions)
Il 10, by Thermo Fisher Scientific (1 mentions)
Tnf α, by Thermo Fisher Scientific (1 mentions)
3 protocols using il 18
1
Quantifying Cellular Biomarkers in Culture
2
Cytokine profile assessment in serum
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The IL-10, IL-6, tumor necrosis factor-α (TNF-α), IFN-α, IFN-β, transforming growth factor-β (TGF-β), and CXCL13 enzyme linked immunosorbent assay (ELISA) kits were purchased from MyBioSource (Vancouver, DC, USA), while the IL-2, IL-17, IL-18, IL-1β, and CXCL1 ELISA kits were purchased from Cloud Clone Corp (Wuhan, China). The level of cytokines in the serum was measured according to the manufacturer’s instructions.
3
Serum Cytokine Profiling by ELISA
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The levels of TNF-α, IL-10 and IL-1β (eBioscience, USA) as well as IL-12, IL-13, IL-15, and IL-18 (Cloud-Clone Corp, CCC, USA) were estimated using enzyme-linked immunosorbent assay (ELISA) kits according to manufacturer's instructions. All coated wells for all determined cytokines were incubated with diluted serum samples as well as two-fold serial dilutions of each standard cytokine. After that, anti-rat antibodies (biotinylated conjugate) were added into each well and incubated for 2 h at room temperature (RT). After period of incubation and washing, streptavidin-HRP (diluted color development enzyme) was added for 1 h at RT. Then, unbound streptavidin was removed by washing followed by tetramethylbenzidine (TMB) substrate incubation in the dark as a color developing agent within 30 min. At the end, the reaction of the enzyme was terminated when a stop solution was added. The formed colored product is proportional to amount of serum cytokine. The absorbance was measured at 450 nm by ELISA reader (Das, Italy). The cytokine concentration was calculated from the standard curve and expressed as pg/ml.
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