Trappc2l

Manufactured by Merck Group

TRAPPC2L is a protein that is part of the TRAPP (Trafficking Protein Particle Complex) family. The TRAPP complex is involved in vesicle tethering and membrane trafficking processes within eukaryotic cells. TRAPPC2L specifically functions as a regulatory subunit of the TRAPP complex, although its exact biological role is not fully characterized.

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Lab products found in correlation

Myc tag, by Abcam (2 mentions) Pp2ac methyl leu 309, by BioLegend (2 mentions) Tacc3, by Santa Cruz Biotechnology (2 mentions) Gm130, by BD (2 mentions) Alexa fluor 488, by Thermo Fisher Scientific (2 mentions) Pp2a scaffold subunit, by Cell Signaling Technology (1 mentions) Ppp2r2a, by Cell Signaling Technology (1 mentions)

2 protocols using trappc2l

Comprehensive Protein Quantification Assay

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Following primary antibodies were used at the indicated dilutions: Myc tag (#ab32, 1:1,000, Abcam), PPP2R1A (#2041, 1:2,500, Cell Signaling Technology), B55α (#5689, 1:2,500, Cell Signaling Technology), B56α (#610615, 1:3000, BD Biosciences), B56δ (#5687, 1:2,500, Cell Signaling Technology), B56ε (#PA5‐17981, 1:2,000, Thermo Fisher Scientific), PP2AC Methyl (Leu 309) (#828801, 1:3,000, BioLegend), PP2AC (#05‐421, 1:4,000, Millipore/Merk), actin, (#sc‐47778 HRP, 1:5,000, Santa Cruz Biotechnology), TRAPPC2L (#HPA041714, 1:2,000, Sigma‐Aldrich), TACC3 (sc‐376883, 1:1,000, Santa Cruz Biotechnology), ROD1 (1:300, a kind gift from Reto Gassmann’s lab), GM‐130 (#610822, 1:500, BD Biosciences), Tubulin (#T9026, DM1A, 1:2,000, Sigma‐Aldrich).

Vit G., Duro J., Rajendraprasad G., Hertz E.P., Holland L.K., Weisser M.B., McEwan B.C., Lopez‐Mendez B., Sotelo‐Parrilla P., Jeyaprakash A.A., Montoya G., Mailand N., Maeda K., Kettenbach A., Barisic M, & Nilsson J. (2022). Chemogenetic profiling reveals PP2A‐independent cytotoxicity of proposed PP2A activators iHAP1 and DT‐061. The EMBO Journal, 41(14), e110611.

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Antibody Validation Protocol

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Following antibodies were used at the indicated dilutions: Myc tag (#ab32, 1:1000, Abcam), PP2A scaffold subunit (#2041, 1:2500, Cell Signaling Technology), PPP2R2A (#5689, 1:2500, Cell Signaling Technology), PPP2R5A (#610615, 1:3000, BD Biosciences), PPP2R5D (#5687, 1:2500, Cell Signaling Technology), PPP2R5E (#PA5-17981, 1:2000, Thermo Fisher Scientific), PP2AC Methyl (Leu 309) (#828801, 1:3000, BioLegend), actin, (#sc-47778 HRP, 1:5000, Santa Cruz Biotechnology), TRAPPC2L (#HPA041714, 1:2000, Sigma-aldrich), TACC3 (sc-376883, 1:1000, Santa Cruz Biotechnology), ROD1 (1:300, a kind gift from Reto Gassmann's lab), GM-130 (#610822, 1:500, BD Biosciences), Alexa Fluor 488 (#A32723, 1:1000, Thermo Fisher Scientific), Tubulin (#T9026, DM1A, 1:2000, Sigma-Aldrich).

Vit G., Duro J., Rajendraprasad G., Hertz E.P.T., Holland L.K., Weisser M., McEwan B.C., López‐Méndez B., Montoya G., Mailand N., Maeda K., Kettenbach A.N., Barišić M., & Nilsson J. (2021). Cellular toxicity of iHAP1 and DT-061 does not occur through PP2A-B56 targeting.

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