Following pH ratiometry of biofilms under static conditions, the buffer capacity of the employed salivary solutions was measured and correlated to the pH drops observed in the biofilms. Each dilution (whole saliva, 1:2, 1:3 and 1:4) was titrated to pH 5.5 with 0.2 M HCl, as measured with a pH electrode (Metrohm AG, Herisau, Switzerland). The buffer capacity (β) was calculated according to equation (2), with n being the amount of HCl in mmol/L:
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β = n Δ p H
Ph electrode
Manufactured by Metrohm
Sourced in Switzerland
The pH electrode is a device used to measure the pH, or acidity, of a solution. It is a core component in various analytical instruments and laboratory equipment. The electrode consists of a glass bulb that is sensitive to the hydrogen ion concentration in the sample, allowing for the accurate determination of the pH value.
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Lab products found in correlation
3 protocols using ph electrode
1
Salivary Buffer Capacity Measurements
2
Potentiometric Copper Ion Sensing
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The pH measurements were done using Titrando 902 with a pH electrode (Metrohm, Swiss) and software tiamo 2.4. The NaOH solution was added automatically by 800 Dosino (Metrohm, Swiss) equipped with a 20‐ml burette. The potential was measured by SevenExcellenceTM (Mettler Tolido, Swiss) with software LabX direct pH 2.4, which can automatically record the potential at an interval of 1 s. A copper ion selective electrode (Bante, U.S.A.) was used to measure potential variation. A magnetic stirrer (IKA, Germany) and a constant temperature water bath apparatus (Guohua Electronic Appliance Co., Ltd., China) were used.
Copper concentration was determined by ICP OES (SPS8000 Bjhaiguang Co., Ltd,) under standard operating conditions.
Copper concentration was determined by ICP OES (SPS8000 Bjhaiguang Co., Ltd,) under standard operating conditions.
3
Measurement of Plutonium in Colloidal Solutions
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The concentration of Pu in colloidal solutions was determined by α-spectrometry (7401 Canberra) performed at the energy of 239 Pu α-decay. The spectrometer was calibrated with sealed sources samples with known activities. Prior the measurement, the samples were diluted and a determined volume was deposited on a stainless steel sample holder and then evaporated by heating. pH determinations were performed with a Metrohm pH electrode, allowing pH measurement on the 0-14 pH range, calibrated thanks to pH = 2.00, 7.00 and 12.45 buffer solutions.
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