All chemicals used were purchased from Fluka chemicals. Their purity was checked by GC. All solvents were purified by distillation using Rotavap (Buchi R120) and if necessary residual water was removed. The components of solvents and elements are given in volume ratios of the components. Methyl gamma linolenate was isolated and purified by using Isolera Flash chromatography System (Biotage INC) and identified using different spectral and HPTLC techniques. The standard methyl gamma linolenate and routine were purchased from Sigma-Aldrich uses thin layer chromatography. The 1H NMR and 13C NMR were recorded on Bruker DRX-300 (300 MHz FT-NMR) using deuterated chloroform as solvent and TMS as internal standard. The mass spectra of compounds were recorded on JEOL GCMATE II GC-MS.
Gcmate 2 gc ms
Manufactured by JEOL
Sourced in United States, Japan
The GCMATE II GC-MS is a gas chromatography-mass spectrometry (GC-MS) system manufactured by JEOL. It is designed to identify and quantify a wide range of chemical compounds through the combination of gas chromatography separation and mass spectrometric detection.
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3 protocols using gcmate 2 gc ms
1
Isolation and Characterization of Methyl Gamma Linolenate
2
GC-MS Analysis of Compound Profiles
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GC-MS analysis of control and treated samples was performed on a GC-MS (JEOL GC MATE II GC-MS, USA), equipped with an integrated gas chromatograph with a HP5 MS column. Helium was used as the carrier gas at a flow rate of 1 mL/min. The injector temperature was 220˚C, with an increase in oven temperature up to 250 ˚C at the rate of 10˚C / min. The compounds were identified by a quadruple double-focusing mass analyzer and photon multiplier tube detector. The mass spectra were analyzed using the National Institute of Structure and Technology (NIST) library.
3
Spectroscopic Characterization of Compounds
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Electron impact-mass spectrometry (EI-MS) was carried out using JEOL GCMATE II GC-MS (Tokyo, Japan) with EI mode of ionization. The Fourier transform-infrared (FT-IR) spectra were recorded using a Perkin-Elmer instrument in the range of 400-4000 cm À1 in thallium bromide. Nuclear magnetic resonance (NMR) spectra were acquired on Bruker Accent multi nuclei probe spectrophotometer at 400 MHz ( 1 H, 13 C). Chemical shifts (d ppm) were referenced with respect to tetra methyl silane (TMS). The spectra ( 1 H, 13 C, DEPT, COSY and HSQC) were recorded in CDCl 3 (Lanos et al. 2012) .
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