Anti igd fitc

Manufactured by Southern Biotech

Sourced in United States

Anti-IgD FITC is a fluorescently labeled antibody that targets the IgD immunoglobulin on the surface of B cells. It is commonly used in flow cytometry applications to identify and characterize B cell populations.

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2 protocols using anti igd fitc

Comprehensive Immunophenotyping of Immune Cells

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Anti-CD19 APC-Cy7, anti-CD38 PerCp-Cy5.5, anti-CD4 PE-Cy7, anti-CD25 PerCP-Cy5.5, anti-CD86 APC, anti-CD80 BV421, anti-CD45RA BV605, anti-ICOS PE, anti-CD21 PE-Cy7, anti-CD80 BV421, anti-CD27 PerCp-Cy5.5, anti-PD-1 APC, anti-CD45RA APC-Cy7, anti-HLA-DR BV605, anti-CD11c AF700, anti-CD86 BV711, anti-CD95 BV650, anti-IgD BV786, anti-FCRL5 APC, anti-CXCR3 BV421, anti-CCR6 BV605, anti-CD19 BV650, anti-CD19 BV605, anti-PD-L1 BV421, anti-PD-L2 PE, anti-ICOS-L PE, anti-OX40L PE, anti-IL-10 PE, anti-IL-10 APC, anti-TNF-α APC-Cy7 (all obtained from BioLegend); anti-IgD FITC and anti-IgD PE (both from Southern Biotech); anti-CD27 PE (Dako); and anti-CD21 PE-Cy7, anti-CD69 FITC, anti-HLA-DR FITC, anti-CD27 BV605, anti-CD40 FITC, anti-CD40L PE, anti-ICAM-1 PE, anti-CXCR5 BUV395 and anti-CD27 BUV395 (all from BD Biosciences).

Reincke M.E., Payne K.J., Harder I., Strohmeier V., Voll R.E., Warnatz K, & Keller B. (2020). The Antigen Presenting Potential of CD21low B Cells. Frontiers in Immunology, 11, 535784.

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Quantifying B Cell Surface Ig Levels

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Single cell suspensions of 0.5−1.0× ×10⁶ bone marrow or red blood cell-depleted spleen cells were incubated with fluorescently labeled antibodies and fixed in 1% paraformaldehyde prior to analysis by flow cytometry. Fluorescent stains include (NIF antibody registry number, where available): anti-CD45R/B220-PE (AB_394620) and –PerCP (AB_394622) (Becton Dickinson- Pharmingen, San Jose, CA, USA), anti-lambda-FTC (AB_609767), anti-kappa-PE (AB_616864), anti-IgM-FITC, anti-IgD-FITC, and anti-CD19-PE/Cy5.5 (Southern Biotech, Birmingham, AL, USA). Stained cells were analyzed using a FACScan analyzer (Becton Dickinson), and results visualized using FlowJo (Treestar, Ashland, OR, USA). Lymphocytes were gated based on FSC and SSC properties. The surface expression level of various Ig was calculated using the mean fluorescence intensity (MFI) of the stained cells, normalized to the MFI of splenic B cells from a non-Tg k+ mouse (either fresh B6 or frozen thawed Balb/c splenocytes, depending on availability).

Clark A.G., Worni-Schudel I.M., Korte F.M, & Foster M.H. (2017). A murine Ig light chain transgene reveals IGKV3 gene contributions to anti-collagen types IV and II specificities. Molecular immunology, 91, 49-56.

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