Anti blt1

Cells were incubated (30 min at 4 °C) with specific or isotype control Abs. ALOX5, ALOX15A and ALOX15B Abs were from Santa Cruz Biotechnology (Dallas, TX, USA), anti‐GPR32 from Acris (Herford, Germany), anti‐BLT1 from LSBio (Seattle, WA, USA), and anti‐ChemR23 and anti‐FPR1 from R&D Systems (Minneapolis, MI, USA). Cells were analysed with a FACSCalibur cytofluorimeter using the cellquest software (BD Biosciences, Lakes, NJ, USA). When necessary, we performed cell membrane permeabilization using the Cytofix/Cytoperm Kit (BD Biosciences). The receptors followed as indicators of resolution responses were the same modulated by FPR1 in the GC model [10 ]. The concentration used for flow cytometric staining was that indicated by manufacturers. AnxA1 staining was performed using a primary anti‐AnxA1 goat polyclonal Ab (1 : 500) (R&D Systems) followed by the staining with a secondary anti‐goat Ab Alexa Fluor 488 (Invitrogen, Waltham, MA, USA). The secondary antibody alone was used as a negative matched control.

Cells were incubated (30 min at 4 °C) with specific or isotype control antibodies (Abs). Anti-ALOX5, ALOX15A, and ALOX15B Abs were from Santa Cruz Biotechnology (Dallas, TX, USA), anti-GPR32 was from Acris (Herford, Germany), anti-BLT1 from LSBio (Seattle, WA, USA), anti-ChemR23 and anti-FPR1 from R&D systems (Minneapolis, MI, USA), anti-TLR7 from Invitrogen (Carlsbad, CA, USA). Cells were analyzed with a FACSCalibur cytofluorimeter using CellQuest software (BD Biosciences). When necessary, we performed cell membrane permeabilization using the Cytofix/Cytoperm kit (BD Biosciences).