Total RNA was extracted from the prepared plant material using an RNAqueous® Total RNA Isolation kit (Thermo Fisher Scientific Baltics UAB, AM1912, Vilnius, Lithuania). The extracted RNAs were first treated with DNaseI using a DNAfree Kit (Thermo Fisher Scientific Baltics UAB, AM1906, Vilnius, Lithuania). RNA quantity was checked using a NanoDrop and quality was tested in 1.5% agarose gel (Sigma-Aldrich, J2500, St. louis, MO, USA). cDNA was synthesized by reverse transcribing 1 μg RNA using a SuperScript II Reverse transcriptase kit (Thermo Fisher Scientific, 18064-014) with anchored-oligo (dT)18 primer (Thermo Fisher Scientific, SO132) according to the manufacturer’s instructions.
J2500
Manufactured by Merck Group
Sourced in United States
The J2500 is a centrifuge designed for general laboratory use. It features a maximum speed of 15,000 RPM and a maximum RCF (Relative Centrifugal Force) of 21,380 x g. The centrifuge can accommodate a variety of rotor options to suit different sample types and volumes.
Automatically generated - may contain errors
Lab products found in correlation
A3903, by Merck Group (2 mentions)
S3007, by Thermo Fisher Scientific (2 mentions)
Dna free kit, by Thermo Fisher Scientific (1 mentions)
Nanodrop, by Merck Group (1 mentions)
Superscript 2 reverse transcriptase kit, by Thermo Fisher Scientific (1 mentions)
Rnaqueous total rna isolation kit, by Thermo Fisher Scientific (1 mentions)
4 protocols using j2500
1
Total RNA Extraction and cDNA Synthesis
2
Phytohormone-Mediated Stress Responses
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Stock solutions were prepared by diluting jasmonic acid (JA; Sigma Aldrich, J2500), 1aminocyclopropanecarboxylic acid (ACC; Sigma Aldrich, A3903) and salicylic acid (SA; Sigma Aldrich, S3007) in absolute ethanol (JA and SA; Fisher Scientific, E/0650DF/17) or dH2O (ACC). Solutions for plant treatments were prepared by diluting stocks with dH2O and supplementing with 0.02% of the surfactant silwet L-77 (LEHLE SEEDS, VIS-30). Pretreatment was performed with 1 mM JA. Challenge consisted of 0.5 mM SA, 0.1 mM JA or 0.1 mM JA + 0.1 mM ACC. The controls for both the pre-treatment ('control') and challenge ('mock') consisted of dH2O supplemented with the same percentage ethanol as in the corresponding hormone solution. Pre-treatments were performed with either 2-week-old seedlings (long-term experiments; 3 weeks prior to challenge) or nearly 5-week-old plants (short-term experiments; 1 day prior to challenge). Challenge treatments were performed when plants were 5 weeks old. All chemical treatments were performed by spraying plants until the leaf surfaces were entirely covered by liquid.
3
Phytohormone-Mediated Stress Responses
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Stock solutions were prepared by diluting jasmonic acid (JA; Sigma Aldrich, J2500), 1aminocyclopropanecarboxylic acid (ACC; Sigma Aldrich, A3903) and salicylic acid (SA; Sigma Aldrich, S3007) in absolute ethanol (JA and SA; Fisher Scientific, E/0650DF/17) or dH2O (ACC). Solutions for plant treatments were prepared by diluting stocks with dH2O and supplementing with 0.02% of the surfactant silwet L-77 (LEHLE SEEDS, VIS-30). Pretreatment was performed with 1 mM JA. Challenge consisted of 0.5 mM SA, 0.1 mM JA or 0.1 mM JA + 0.1 mM ACC. The controls for both the pre-treatment ('control') and challenge ('mock') consisted of dH2O supplemented with the same percentage ethanol as in the corresponding hormone solution. Pre-treatments were performed with either 2-week-old seedlings (long-term experiments; 3 weeks prior to challenge) or nearly 5-week-old plants (short-term experiments; 1 day prior to challenge). Challenge treatments were performed when plants were 5 weeks old. All chemical treatments were performed by spraying plants until the leaf surfaces were entirely covered by liquid.
4
Effects of Jasmonic Acid on Hemp Hypocotyl Growth
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Hemp (Cannabis sativa L.) hypocotyls of the fibre cv Santhica 27 were grown in a mixture of compost/sand (1:1, w/w) in controlled conditions (Behr et al. 2016) (link). Seeds were provided by the Coopérative Centrale des Producteurs de Semences de Chanvre (Beaufort-en-Vallée, France) and certified by the Service Officiel de Contrôle et de Certification (SOC) of the Groupement National Interprofessionnel des Semences et plants (GNIS), France. Either JA, at a concentration ranging from 0.03 to 3 mM, or the mock solution was sprayed on plantlets aged 15 days after sowing (1 mL plant -1 ). The JA solution (Sigma-Aldrich, J2500) was prepared in phosphate buffer saline (PBS) from a 500fold stock in pure ethanol. A mock solution, consisting of PBS with a minute amount of ethanol (0.06% in the sprayed solution) compensating for its presence in the jasmonic acid solution, was sprayed on control plantlets.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!