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5 bromo 2 deoxyuridine brdu labeling reagent

Manufactured by Roche
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5-Bromo-2′-deoxyuridine (BrdU) Labeling reagent is a synthetic thymidine analog that can be incorporated into the DNA of dividing cells during the S-phase of the cell cycle. The presence of BrdU can be detected using specific antibodies, allowing for the identification and quantification of proliferating cells.

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Lab products found in correlation

Brdu labeling and detection kit, by Roche (2 mentions) Alkaline phosphatase labeled goat anti rabbit igg antibody, by Merck Group (1 mentions) Anti akt antibody, by Cell Signaling Technology (1 mentions) Goat anti mouse igg antibody, by Merck Group (1 mentions) 96 well culture plate, by Thermo Fisher Scientific (1 mentions) Pdgf bb, by Thermo Fisher Scientific (1 mentions) Cell proliferation elisa brdu kit, by Roche (1 mentions)

4 protocols using 5 bromo 2 deoxyuridine brdu labeling reagent

1

Cell Proliferation and Signaling Assays

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Sp was purchased from Sigma-Aldrich (St. Louis, MO, USA). 5-Bromo-2′-deoxyuridine (BrdU) Labeling reagent and the Detection Kit I were obtained from Roche Diagnostics GmbH (Mannheim, Germany). Anti-cyclin D1 and anti-p27 antibodies were from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA). Anti-mitogen-activated protein kinase (MAPK), anti-PI3K p85 and anti-AKT antibodies were all purchased from Cell Signaling Technology, Inc. (Danvers, MA, USA). Alkaline phosphatase-labeled goat anti-rabbit IgG antibody and goat anti-mouse IgG antibody were both from Sigma-Aldrich.
WEI C., LI H.Z., WANG Y.H., PENG X., SHAO H.J., LI H.X., BAI S.Z., LU X.X., WU L.Y., WANG R, & XU C.Q. (2015). Exogenous spermine inhibits the proliferation of human pulmonary artery smooth muscle cells caused by chemically-induced hypoxia via the suppression of the ERK1/2- and PI3K/AKT-associated pathways. International Journal of Molecular Medicine, 37(1), 39-46.
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2

Measuring Osteoblast and Osteocyte Proliferation

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To detect the extent of cell proliferation in the osteoblast and osteocyte populations, 5′-bromo-2′deoxyuridine (BrdU) labeling reagent (45 μg g−1 body weight; Roche, Indianapolis, IN, USA) was injected intraperitoneally into mice at P10. Two hours after injection, their femora were dissected, embedded and sectioned in the mid-sagittal plane for immunodetection with a BrdU labeling and detection kit (Roche). For statistical analyses, three independent littermates were used in each of the studies.
Moon Y.J., Yun C.Y., Choi H., Ka S.O., Kim J.R., Park B.H, & Cho E.S. (2016). Smad4 controls bone homeostasis through regulation of osteoblast/osteocyte viability. Experimental & Molecular Medicine, 48(9), e256-.
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3

Evaluation of Mesangial Cell Proliferation

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Primary human mesangial cells were grown to near confluence in 96-well culture plates (Nunc) using CS-C complete serum free medium and kept in resting condition as described above. Cells were left unstimulated or incubated with stimulants suspended in CS-C serum-free-maintenance medium containing 5-bromo-2′-deoxyuridine (BrdU)-labeling reagent (100 μM, Roche Diagnostics) for 24 h. Stimulants included galactose-deficient polymeric IgA1-IgAN (100 μg/mL), M4 or M4Δ451 (both at 10 μg/mL) as well as M4 and M4Δ451 pre-incubated for 1 h with galactose-deficient polymeric IgA1-IgAN. The positive control was murine platelet-derived growth factor (PDGF)-BB (50 ng/ml, PeproTech Inc, Rocky Hill, NJ). Proliferation was detected using a calorimetric BrdU cell proliferation ELISA-kit (Roche Diagnostics) according to manufacturer's instructions.
Schmitt R., Ståhl A.L., Olin A.I., Kristoffersson A.C., Rebetz J., Novak J., Lindahl G, & Karpman D. (2014). The combined role of galactose-deficient IgA1and streptococcal IgA-binding M protein in inducing IL-6 and C3 secretion from human mesangial cells: implications for IgA nephropathy. Journal of immunology (Baltimore, Md. : 1950), 193(1), 317-326.
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4

Detecting Cell Proliferation in Developing Roots

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To detect cell proliferation in developing roots, 5'-bromo-2' deoxyuridine (BrdU) labeling reagent (45 µg/g body weight; Roche, Indianapolis, IN, USA) was injected intraperitoneally into 10-day-old wild type (WT) and mutant (MT) mice. Two hours after injection, mice were dissected, the mandibles were fixed with Carnoy's fixatives at 4℃ overnight, embedded in paraffin, and sectioned in the root elongation region and furcation region for immunodetection with the BrdU labeling and detection kit (Roche). For statistical analysis, 5 consecutive sections from 3 independent littermates were used.
Kim T.H., Bae C.H., Yang S., Park J.C, & Cho E.S. (2015). Nfic regulates tooth root patterning and growth. Anatomy & Cell Biology, 48(3), 188-194.
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