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4 protocols using tefose 63

1

Voriconazole Bioavailability Enhancement Formulations

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Voriconazole (VCZ) was provided by Pharco pharmaceuticals (Alexandria, Egypt) as a kind gift. Estradiol valerate was bought from Sigma Aldrich (Germany). Maisine® CC (Glyceryl mono-linoleate), Tefose® 63 (Glycol stearate PEG-6 stearate PEG-32 stearate, HLB = 9.5), and Gelot™ 64 (Glyceryl stearate and PEG-75 stearate, HLB = 10) were obtained from Gattefossé (St-Priest, France). Glyceryl monostearate (GMS), Glyceryl monooleate (GMO), and Sterotex®NF (Hydrogenated vegetable oil, type I) were purchased from Abitec Corporation (Columbus, OH). Tween® 80, cellulose membrane tubing (molecular weight cutoff 14,000 Daltons), and mucin were acquired from Sigma-Aldrich, USA. Sodium acetate, glacial acetic acid, and agar were supplied by El-Nasr pharmaceutical chemicals Co. (Cairo, Egypt). Any used chemicals and reagents were of standard grade.
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2

Lipid-based Nanoparticle Formulation Development

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MF was gifted by Centaur Pharmaceuticals, Mumbai. Glycerol monostearate (GMS), Compritol 888 ATO, Apifil, Tefose-63 and Emulcire were gifted by Gattefosse India Pvt. Ltd., Mumbai. Cetyl palmitate, Syncrowax-HRC, Syncrowax-HGL were gifted by Croda Chemicals, Mumbai. Stearic acid, Tween-80 from Lubrizol, Mumbai and Phospholipon-80 H, Lipoid S-75 (Soya Lecithin) from Lipoid, Germany were gift samples. Ethanol, Acetone and Carbopol 974p were purchased from Research Lab Fine Chem., industries, Mumbai. Methyl paraben, Propyl paraben, Triethanolamine were purchased from Loba Chemie Pvt. Ltd, Mumbai.
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3

Chrysomycin A Purification and Formulations

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Chrysomycin A (≥95%) could be obtained by fermentation, subsequent extraction, and purification according to our previous report [18 (link)]. Tefose® 63 (Mixture of Polyoxyl 6 Stearate Type I, Ethylene Glycol Stearates, and Polyoxyl 32 Stearate Type I) and Labrifil® M 1944 CS (Oleoyl polyoxyl-6 glycerides) were purchased from GATTEFOSSÉ (Saint Priest, France). Formic acid, methanol, dimethyl sulfoxide (DMSO), 2-(2-Ethoxyethoxy) ethanol, 1-octadecanol, 1,2-propanediol, and benzoic acid were provided by Sinopharm Chemical Reagent limited corporation (Beijing, China). Fucidin, vancomycin hydrochloride and mupirocin powder were purchased from Aladdin Bio-Chem Technology Co., Ltd. (Shanghai, China); 1% Erythromycin ointment was provided by Chongqing Kerui Pharmaceutical (Group) Co., Ltd. (Chongqing, China), 2% mupirocin ointment (Bactroban®) was provided by Tianjin Smith & French laboratories Ltd., China.
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4

Topical Curcumin Delivery System

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Labrasol (caprylocaproyl polyoxyl-8 glyceride), Tefose 63 (mixture of PEG-6 stearate, ethylene glycol palmitostearate and PEG-32 stearate), and Sedefos 75 (mixture of triceteareth-4 phosphate, ethylene and diethylene glycol stearate) were obtained from Gattefossé (Lyon, France). Cremophor RH 40 (polyoxyl 40 hydrogenated castor oil) was obtained from BASF Company (Ludwigshafen, Germany). Pemulen TR-1 and Carbopol 974P were purchased from Lubrizol Corporation, Ohio, USA. Stearic acid, cetylstearyl alcohol, propylene glycol, and isopropyl myristate were purchased from Hungaropharma Ltd., (Budapest, Hungary). The MTT dye, curcumin, DPPH free radical, human and rat TNF-α and IL-1β ELISA Kits and ingredients needed for the cell maintenance were purchased from Sigma-Aldrich (Budapest, Hungary). The immortalized human keratinocyte cell line (HaCaT) was obtained from Cell Lines Service (CLS, Heidelberg, Germany).
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