α asf1a

Manufactured by Cell Signaling Technology

α-ASF1A is an antibody that recognizes the ASF1A (Anti-silencing function 1A) protein. ASF1A is a histone chaperone involved in chromatin assembly and disassembly processes.

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Lab products found in correlation

α gapdh, by Proteintech (1 mentions) α flag, by Cell Signaling Technology (1 mentions) Ab124971, by Abcam (1 mentions) P0447, by Agilent Technologies (1 mentions)

Spelling variants of this product

ASF1A (4 citations)

2 protocols using α asf1a

Comprehensive Antibody Panel for Chromatin Biology

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α-H3 (# 4499; 1:1000), α-FLAG (# 2368; 1:1000), and α-ASF1A (# 2990; 1:500) were purchased from Cell Signaling; α-HDAC1 (# 05-100-1; 1:500) was obtained from Millipore; α-p300 (# sc-584; 1:250) was obtained Santa Cruz Biotechnology; α-CBP (# GTX101249; 1:500) and α-PCAF (# GTX109666; 1:500) were obtained from GeneTex; α-CAF-1 p48 (# ab47456; 1:500), α-CHAF1B (# ab72520; 1:500), α-CHAF1A (# ab126625; 1:1000) and α-XRCC1 (# 134056; 1:1000) were obtained from Abcam; α-DNA Pol β (# 18003-1-AP; 1:1000), α-βactin (# 66009-1-Ig; 1:3000), α-GAPDH (# 60004-1-Ig; 1:3000) were obtained from Proteintech. α-PNKP [35 (link)] (1:500) was a kind gift from Dr. Michael Weinfeld (Cross Cancer Institute, University of Alberta, Canada) and α-NEIL1 [27 (link)] (1:500) was custom-generated. α-AcNEIL1 (1:500) was custom generated through EZBiolab using a chemically synthesized NEIL1 peptide (²⁸⁸APKGRKSRK*K*K*SKA³⁰¹) containing acetylated Lys (*) as a hapten to elicit IgG antibody in rabbit and purified the IgG from the sera. All antibodies were diluted in 5% non-fat dry milk in tris-buffered saline (TBS) containing 0.05% Tween20.

Sengupta S., Yang C., Hegde M.L., Hegde P.M., Mitra J., Pandey A., Dutta A., Datarwala A.T., Bhakat K.K, & Mitra S. (2018). Acetylation of oxidized base repair-initiating NEIL1 DNA glycosylase required for chromatin-bound repair complex formation in the human genome increases cellular resistance to oxidative stress. DNA repair, 66-67, 1-10.

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Comprehensive Protein Expression Analysis

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Western blot was performed as described⁴². Primary antibodies were α-RAD52 (Abcam ab124971, 1:2,000), α-BLM (Abcam ab2179, 1:1,000), α-PolD3 (Abcam ab182564, 1:5,000), α-SLX4 (kind gift by Prof. John Rousse, 1:5,000), α-ATRX (Bethyl A301-045A, 1:500), α-DAXX (Santa Cruz sc7152, 1;1,000), α-H3 (Abcam ab10799, 1:2,000), α-ASF1a (Cell Signalling 2990, 1:1,000), α-LANA (Millipore MABE1109, 1:1,000), α-ORF57 (Santa Cruz sc135746 1:1,000), α-beta actin (Biolegend 664802 1;2,000). Secondary HRP-coupled secondary antibodies were α-mouse (Dako P0447 1:2,000), α-rabbit (Dako P0399 1:2,000), α-rat (Abcam ab97057 1:2,000). Uncropped blots are available in Source data file.

Lippert T.P., Marzec P., Idilli A.I., Sarek G., Vancevska A., Bower M., Farrell P.J., Ojala P.M., Feldhahn N, & Boulton S.J. (2021). Oncogenic herpesvirus KSHV triggers hallmarks of alternative lengthening of telomeres. Nature Communications, 12, 512.

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