Sabouraud dextrose liquid medium

Manufactured by Thermo Fisher Scientific

Sabouraud dextrose liquid medium is a culture medium used for the growth and isolation of fungi. It provides the necessary nutrients and growth factors to support the cultivation of a variety of fungal species.

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Lab products found in correlation

Pluronic f 127, by Merck Group (1 mentions) Propidium iodide, by Merck Group (1 mentions) L glutamine, by Merck Group (1 mentions) Methylene blue, by Biomaxima (1 mentions) Mueller hinton lab agar, by Biomaxima (1 mentions) Tryptic soy broth (tsb), by Biomaxima (1 mentions) Rpmi 1640 medium, by Merck Group (1 mentions) Rt hs pcr mix probe, by A&A Biotechnology (1 mentions) Nile red, by Santa Cruz Biotechnology (1 mentions)

Spelling variants of this product

Dextrose (94 citations) Sabouraud medium (8 citations) Sabouraud liquid medium (3 citations) Sabouraud Dextrose medium (2 citations) Sabouraud Dextrose Liquid Medium (SDLM; (2 citations)

4 protocols using sabouraud dextrose liquid medium

Antifungal Activity Assay Protocol

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Pluronic F-127, RPMI-1640 medium with the addition of l-glutamine and without
sodium bicarbonate, PBS (phosphate-buffered saline), propidium iodide, and amphotericin B
were purchased from Sigma-Aldrich (USA). Nile red was obtained from Santa Cruz
Biotechnology (USA). Fluconazole and verapamil hydrochloride were obtained from Pol-Aura
(Poland). Sterile, purified water was purchased from Polpharma (Poland).
3-morpholinopropanesulfonic acid (MOPS) was purchased from J&K Scientific (China).
Mueller Hinton LAB-AGAR with glucose and methylene blue were both purchased from Biomaxima
(Poland). Sabouraud dextrose agar with chloramphenicol was purchased from Liofilchem
(Italy). Sabouraud dextrose liquid medium was purchased from Oxoid (Great Britain).
Tryptic soy broth was bought from Biomaxima (Poland). Zymo Research—YeaStar RNA Kit
was obtained from TK Biotech (Poland). The primers were ordered from Genomed (Poland). The
TranScriba Kit and the RT HS-PCR Mix Probe were supplied by A&A Biotechnology
(Poland).

Malec K., Mikołajczyk A., Marciniak D., Gawin-Mikołajewicz A., Matera-Witkiewicz A., Karolewicz B., Nawrot U., Khimyak Y.Z, & Nartowski K.P. (2023). Pluronic F-127 Enhances the Antifungal Activity of Fluconazole against Resistant Candida Strains. ACS Infectious Diseases, 10(1), 215-231.

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Cultivation of B. caledonica from Pine Tree

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2.1. B. caledonica strain and growth conditions B. caledonica 2c7b isolated from the stump of a felled pine tree (Hortland, Co. Kildare, Ireland), was used in this study.
B. caledonica was cultured in Sabouraud dextrose liquid medium (Oxoid) for 96 h in a shaking incubator at 25 C, 250 rpm. The culture supernatant was filtered through 0.22 mm syringe filters (Sarstedt). The supernatant was collected and stored at À80 C.

Mc Namara L., Dolan S.K., Walsh J.M.D., Stephens J.C., Glare T.R., Kavanagh K, & Griffin C.T. (2019). Oosporein, an abundant metabolite in Beauveria caledonica, with a feedback induction mechanism and a role in insect virulence. Fungal biology, 123(8).

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Isolation and Cultivation of Entomopathogenic Fungi

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A commercial strain of M. brunneum (Met52; previously M. anisopliae) produced by Novozymes (Denmark) was used and was purchased on rice grains from National Agrochemical Distributors, Lusk, Dublin. B. bassiana experimental strain 1694 was supplied by Becker Underwood (Littlehampton, UK). B. caledonica (2c7b) is a native strain isolated from a soil sample from soil close to a pine stump in a felled forest in Hortland, Co. Kildare (Ireland). The soil sample was baited with G. mellonella larvae and fungus from the infected cadaver was identified through DNA sequencing of an ITS PCR product (a region of the internal transcribed spacer unit of the ribosomal DNA, ITS4, was amplified by PCR). EPF were cultured in Sabouraud Dextrose liquid medium (Oxoid) for 48 h, 72 h and 96 h in a shaking incubator at 25°C and 250 rpm. After each time point the culture was filtered through 0.45 µm syringe filters and then through 0.2 µm syringe filters (Sartstedt). The filtrate was collected and stored at -80 °C.

Namara L.M., Griffin C.T., Fitzpatrick D., Kavanagh K, & Carolan J.C. (2018). The effect of entomopathogenic fungal culture filtrate on the immune response and haemolymph proteome of the large pine weevil, Hylobius abietis. Insect biochemistry and molecular biology, 101.

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Isolation and Cultivation of Entomopathogenic Fungi

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A commercial strain of M. anisopliae (Met52) produced by Novozymes (Denmark) was used. Met 52 was purchased on rice grains from National Agrochemical Distributors, Lusk, Dublin. B. bassiana experimental strain 1694, was supplied by Becker Underwood (Littlehampton, UK). B. caledonica (2c7b) is a native strain isolated from a soil sample from soil close to a pine stump in a felled forest in Hortland, Co. Kildare (Ireland). The soil sample was baited with G. mellonella larvae and fungus from the infected cadaver was identified through DNA sequencing of an ITS PCR product (a region of the internal transcribed spacer unit of the ribosomal DNA, IST4, was amplified by PCR). EPF were cultured in Sabouraud Dextrose liquid medium (Oxoid) for 48 h, 72 h and 96 h in a shaking incubator at 25 °C and 250 rpm. After each time point the culture was filtered through 0.45 µm syringe filters and then through 0.2 µm syringe filters (Sartstedt). The filtrate was collected and stored at -80 °C.

Mc Namara L., Carolan J.C., Griffin C.T., Fitzpatrick D, & Kavanagh K. (2017). The effect of entomopathogenic fungal culture filtrate on the immune response of the greater wax moth, Galleria mellonella. Journal of insect physiology, 100.

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