All animal experiments were approved by the University of Pittsburgh Institutional Animal Care and Use Committee. All procedures were carried out in accordance with the approved guidelines. APP/PS1ΔE9 mice were bred to targeted replacement APOE3+/+ and APOE4+/+ mice to generate APP/PS1ΔE9/APOE3+/+ and APP/PS1ΔE9/APOE4+/+ (referred to as APP/E3 and APP/E4, respectively) [23 (link)]. Males and females were randomly assigned to Normal diet (ND, Prolab Isopro RMH 3000, Lab Diet) or HFD (D12079B RD Western Diet, Research Diets) at a mean age of 3.5 months and fed for a 3 month period. HFD (D12079B RD Western Diet, Research Diets) supplements 40% calories from fat (fat was provided as anhydrous milk fat and corn oil), 16.8% from protein, and 42.6% from carbohydrates. ND (Prolab Isopro RMH 3000, Lab Diet) supplements 14.3% from fat, 26.0 % from protein, and 59.65 % from carbohydrates.
D12079b rd western diet
Manufactured by Research Diets
The D12079B RD Western Diet is a laboratory diet product offered by Research Diets. It is designed for use in research studies. The diet's core function is to provide a standardized, semi-purified diet formulation.
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Lab products found in correlation
2 protocols using d12079b rd western diet
1
Dietary Effects on Alzheimer's Mouse Model
2
High-Fat Diet Exacerbates Alzheimer's in APP23 Mice
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All animal experiments were approved by the University of Pittsburgh Institutional Animal Care and Use Committee. All procedures were carried out in accordance with the approved guidelines. APP23 transgenic mice (C57Bl6 background) expressing human APP751 familial Swedish AD mutation (APPK670N, M671L) were crossed to wild-type C57BL/6 mice to generate APP23 hemizygous mice53 (link). Male and female APP23 mice (mean age 11.7 months) were randomly assigned to ND (Prolab Isopro RMH 3000, Lab Diet) or HFD (D12079B RD Western Diet, Research Diets). HFD supplemented 40% calories from fat; 16.8% from protein and 42.6% from carbohydrates. Fat was provided as anhydrous milk fat and corn oil. The mice had free access to water and food. After 3 months of feeding, behavior was assessed at mean age of 14.7 months.
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