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Alexa fluor 488 conjugated anti cxcr5

Manufactured by BD
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Alexa Fluor 488-conjugated anti-CXCR5 is a fluorescently labeled antibody that specifically binds to the CXCR5 chemokine receptor. This product is intended for use in flow cytometry, immunofluorescence, and other applications where the detection of CXCR5-expressing cells is required.

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Lab products found in correlation

Pe cy5 conjugated anti cd3, by Thermo Fisher Scientific (2 mentions) Pe conjugated anti slam, by Thermo Fisher Scientific (2 mentions) Pe conjugated anti sap, by Thermo Fisher Scientific (2 mentions) Cellquest software, by BD (2 mentions) Fitc conjugated anti cd19, by BioLegend (2 mentions) Facscalibur flow cytometer, by BD (1 mentions) Apc conjugated anti cd4, by BioLegend (1 mentions) Facscalibur ow cytometer, by BD (1 mentions)

2 protocols using alexa fluor 488 conjugated anti cxcr5

1

Multicolor Flow Cytometry of Immune Cells

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Cells were washed and immunostained with phycoerythrin (PE)-Cy5-conjugated anti-CD3, PE-conjugated anti-SLAM, PE-conjugated anti-SAP (eBioscience, San Diego, CA), allophycocyanin-conjugated anti-CD4, FITC-conjugated anti-CD19 (BioLegend, San Diego, CA), and Alexa Fluor 488-conjugated anti-CXCR5 (Becton Dickinson, San Jose, CA) mAbs against the human cell surface. Isotype-matched Ab controls were used in all procedures. The entire staining process was performed according to the manufacturer’s protocol. The stained cells were analyzed on a FACSCalibur flow cytometer with CELLQUEST software (Becton Dickinson, Sparks, MD).
Yang J., Geng L., Ma Y., Tang X., Peng H., Tian J., Xu H, & Wang S. (2021). SLAMs Negatively Regulate IL-21 Production in Tfh-Like Cells from Allergic Rhinitis Patients. Journal of Asthma and Allergy, 14, 361-369.
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2

Immunophenotyping of Human Lymphocytes

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Cells were washed and immunostained with PE-Cy5-conjugated anti-CD3, PE-conjugated anti-SLAM, PEconjugated anti-SAP (eBioscience, San Diego, CA), APC-conjugated anti-CD4, FITC-conjugated anti-CD19 (BioLegend, San Diego, CA), and Alexa Fluor 488-conjugated anti-CXCR5 (Becton Dickinson, San Jose, CA) mAbs against the human cell surface. Isotype-matched Ab controls were used in all procedures. The whole staining process was performed according to the manufacturer's protocol. The stained cells were analyzed on a FACSCalibur ow cytometer with CELLQUEST software (Becton Dickinson, Sparks, MD).
Yang J., Geng L., Ma Y., Tang X., Peng H., Tian J., Xu H., & Wang S. (2020). SLAM Negatively Regulates IL-21 Production in Tfh-Like Cells from Allergic Rhinitis Patients.
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