Goat anti pericentrin 2

The following primary antibodies were used: rabbit anti-Nogo-A/RTN4 (AHP1799, Biorad, Feldkirchen, Germany), mouse anti-TOM20 (Santa Cruz, CA, USA, sc-17764), mouse anti-SIGMA1R (Santa Cruz, sc-137075), rat anti-SIGMA1R (Merck, Darmstadt, Germany, ICR-SIG1R-A), mouse anti-ACSL4 (Santa Cruz, sc-365230), mouse anti-Myc (Cell Signaling, Danvers, MA, USA, 2276), rabbit anti-HA (Sigma-Aldrich, St. Louis, MO, USA, H6908), rat anti-HA (Roche, Basel, Switzerland, 3F10), rabbit anti-VLGR1, raised against the against the C-terminus of murine VLGR1 (amino acids 6198–6307) and previously characterized in [9 (link),14 (link)], rabbit anti-α-Tubulin (Abcam, Cambridge, UK, DM1A), rabbit anit-COX IV (NEB, Ipswich, MA, USA, 3E11), rabbit anti-GFAP (Dako Agilent, Santa Clara, CA, USA, Z0334), rat anti-RFP (Chromotek, Planegg, Germany, 5F8), mouse anti-CLIMP63, (Enzo Life Sciences, G1/296), goat anti-Pericentrin 2 (Santa Cruz, C-16), mouse anti-Arl13b (Abcam, Cambridge, UK, ab136648), goat anti-Centrin 2 [15 (link)]. Secondary antibodies conjugated Alexa 488, Alexa 555, Alexa 568 or Alexa 647 were purchased from Molecular Probes (Life Technologies, Darmstadt, Germany) or Rockland Inc. (Gilbertsville, PA, USA). Nuclear DNA was stained with DAPI (4′,6-diamidino-2-phenylindole, 1 mg/mL: Sigma-Aldrich).

The following antibodies were used: rabbit anti-VLGR1 [20 (link)], mouse anti-Vinculin (Merck KGaA, Darmstadt, Germany, clone hVIN-1), mouse anti-CLIMP63 (Enzo Life Sciences, Lörrach, Germany, G1/296), rat anti-HA (Roche, clone 3F10), mouse anti-FLAG M2 (Merck KGaA, clone M2), rabbit anti-GFP (kind gift from Dr. Clay Smith, University of Florida), mouse anti-α-Tubulin (Abcam, Cambridge, UK, clone DM1A), and goat anti-Pericentrin 2 (Santa Cruz Biotechnology, Dallas, TX, USA, clone C-16). Secondary antibodies conjugated to Alexa 488, Alexa 568, or Alexa 647 were purchased from Molecular Probes (Life Technologies, Darmstadt, Germany) or from Rockland Inc. (Gilbertsville, PA, USA). Nuclear DNA was stained with DAPI (1 mg/mL) (Merck KGaA).