E el m0021c

Male C57 mice were randomly divided into seven groups and fasted for 12 h before the experiment. The mice in the model group underwent intraperitoneal injection with LPS (5 mg/kg) to induce a cytokine storm, while the mice in the blank control group were injected with the same volume of normal saline. The eyeballs of the mice were removed and blood samples were collected at 1.5, 4, 6, 8, 12, and 24 h after the administration of LPS. Blood samples were placed in tubes containing heparin sodium. Then, the blood samples were centrifuged for 10 min at 1000g. The supernatant was extracted and stored at −80°C. The concentrations of IL‐1α, IFN‐γ, TNF‐α, MCP‐1, IL‐12p70, IL‐10, IL‐27, IL‐23, IL‐17A, and IFN‐β in the plasma were measured using the Mouse Inflammation Panel (Cat. no. 740446, BioLegend). And MIG, MIP‐1α, MIP‐1β, and IL‐4 were measured using the Mouse Cytokine Release Syndrome Panel (Cat. no.741024, BioLegend). IL‐6 was measured using the ELISA (Cat. no. E‐EL‐M0021c, Elabscience).

Cytokine levels in mouse plasma and prostate tissue homogenate samples from immunized mice were detected using ELISA kits for CXCL9 (E-EL-M0020c, Elabscience, Wuhan, China), CXCL10 (E-EL-M0021c, Elabscience, Wuhan, China), CXCL11 (JYM1057Mo, Elisa Lab, Wuhan, China), IFN-γ (E-EL-M0048c, Elabscience, Wuhan, China), IL-6 (CSB-E04639m, Cusabio, Wuhan, China), and MCP1 (CSB-E07430m, Cusabio, Wuhan, China). Serum cytokine levels for CP/CPPS patients were assessed using ELISA kits for CXCL9 (E-EL-H6062, Elabscience, Wuhan, China), CXCL10 (E-EL-H0050c, Elabscience, Wuhan, China), and CXCL11 (E-EL-H0051c, Elabscience, Wuhan, China).