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3 protocols using 3 aminopropyl triethoxysilane

1

Synthesis and Characterization of CTAB-Chitosan Nanoparticles

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Hexadecyltrimethylammonium bromide (CTAB, 99%), (3-aminopropyl)triethoxysilane (APTES, 98%), chitosan, superdry tetrahydrofuran (THF, 99.5%) and N,N-diisopropylethylamine (DIPEA, 99%) were purchased from J&K Chemical. Cyanuric chloride (CYC, 99%) was purchased from Energy Chemical. Bergamot oil and lemon oil were purchased from Shanghai Yuanye Bio-Technology Co.,Ltd. Ammonium hydroxide (AR, 25−28%), hydrogen chloride (HCl, AR, 36−38%), ethanol (AR), acetone (AR), dimethylsulfoxide (AR, DMSO), cyclohexane (AR), methanol (AR) and dichloromethane (DCM) were obtained from Sinopharm Chemical Reagent Co.,Ltd. CORT (97%) was purchased from TCI.
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2

Synthesis of Fluorescent Silica Nanoparticles

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Tetraethoxysilane (TEOS,A.R), ammonia solution(NH3·H2O) (25~28%, A.R), and sodium carbonate anhydrous(Na2CO3,A.R) were obtained from Shanghai Lingfeng Chemical Reagent Co.LTD. Ethyl alcohol absolute (EtOH) was obtained from Shanghai Zhenxing No.1 Chemical Plant. 3-Aminopropyltriethoxysilane (98%, APTES) was purchased from J&K Scientific LTD. Deionized water was used in all experiments. Cetyltrimethyl Ammonium Bromide (CTAB) was purchased from Sigma-Aldrich. Fluorescein isothiocyanate isomeric (FITC, 90%) was purchased from ACROS ORGANICS.
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3

Synthesis and Characterization of Mesoporous Silica Nanoparticles

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Cetyltrimethylammonium chloride (CTAC), trimethylamine (TEA) and cyclohexane were purchased from Shanghai Lingfeng Chemical Reagent Co. Ltd. China. Tetraethylorthosilicate (TEOS) was obtained from Sinopharm Chemical Reagent Co. Ltd. China. 3-Aminopropyltriethoxysilane (APTMS) was purchased from J&K Scientific Co. Ltd. China. α-Methyl-4-(isobutyl)phenylacetic acid (IBU) was bought from Alfa Aesar Co. Ltd. China. N-Hydroxysuccinimide (NHS) and 1-ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride (EDC) were obtained from Sigma Aldrich Chemicals Co. Ltd. China. Ammonium-nitrate (NH4NO3) was received from Xilong Scientific Co. Ltd. China. Decahydronaphthalene was purchased from Aladdin Co. Ltd. China. Dimethyl formamide (DMF) and dimethyl sulphoxide (DMSO) were bought from Shanghai Titan Scientific Co. Ltd. China. Bovine serum albumin (BSA) was obtained from Shanghai Yeasen Biotechnology Co. Ltd. China. 3-(4,5-Dimethyl-2-thiazolyl)-2,5-di-phenyl-2-tetrazolium bromide (MTT) was received from Life Technology. USA. The chemicals used were of analytical grade and without further purification.
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