Anti p mtor ab109268

SA and 5-aminosalicylic
acid (5ASA) were purchased from Aladdin Chemicals Co., Ltd. (Shanghai,
China). The standard AIN-93G diet was purchased from SYSE Biotechnology
Co., Ltd. (Changzhou, China). DSS was obtained from MP Biomedicals
(Santa Ana, USA). Carboxymethyl cellulose (CMC), the H&E staining
kit, Masson’s trichrome staining solution, and the primary
antibodies (Caspase1, LC3, β-actin) were acquired from Servicebio
Biotechnology Co., Ltd. (Wuhan, China). Anti-ASC (AF6234), anti-α-SMA
(AF1507), anti-Beclin1 (AF5123), anti-p62 (AF5312), anti-Nrf2 (AF7623),
anti-p-Nrf2 (AF1609), anti-Keap1 (AF7335), anti-AMPK (AF1627), anti-p-AMPK
(AF2677), anti-Akt (AF1777), anti-p-Akt (AF1546), and anti-mTOR (AF1648)
antibodies were acquired from Beyotime Biotechnology Co., Ltd. (Shanghai,
China). Anti-NLRP3 (ab263899), anticollagen-I (ab270993), and anti-p-mTOR
(ab109268) were obtained from Abcam (Cambridge, MA, USA). Anti-IL-1β
(PTR2541) was acquired from ImmunoWay Biotechnology Co., Ltd. (Jiangsu,
China).

Isobaric tags for relative and absolute quantitation (iTRAQ) Reagent Multiplex Kit (4352135) were purchased from AB Sciex (Foster City, CA). Biotin-Streptavidin HRP Detection Systems and Diaminobenzidine (DAB) Kit (ZLI-9018, China) were from ZSGBBio Company (Beijing, China). Acetonitrile (14261) and formic acid (56302) were from Sigma-Aldrich. Anti-ANGPT2 (ab155106), anti-SPP1 (ab214050), anti-ERK (ab17942), anti-pERK (ab214362), anti-mTOR (ab2732), anti-pmTOR (ab109268), and anti-PI3K (ab40776) were from Abcam.