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Pcdna3.1 hygro gja4 cx37 cloneid

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PcDNA3.1/Hygro(+)-GJA4 (Cx37; cloneID) is a plasmid vector that contains the coding sequence for the Gap junction alpha-4 protein (GJA4), also known as Connexin 37 (Cx37). This plasmid is designed for expression of the Cx37 protein in mammalian cell lines.

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Lab products found in correlation

Cx46 cdna, by R&D Systems (2 mentions) Pcdna3.1 hygro gjc1 cx45 cloneid, by GenScript (2 mentions) Plpcx cx43 ires gfp, by Addgene (2 mentions)

Spelling variants of this product

PcDNA3.1 (90 citations) PcDNA3.1/Hygro (3 citations)

2 protocols using pcdna3.1 hygro gja4 cx37 cloneid

1

Engineered Connexin Expression Constructs

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The Cx46 expression vector was created by inserting the Cx46 cDNA (catalog# RDC0535, R&D Systems) between the HindIII and XbaI sites of pEGFP-N3, excising the GFP tag. This backbone was used for site-directed mutagenesis to introduce the L11S, T19M, and cysless mutations, using the primers shown in Table S2. The primers for the cysless mutant were designed so that the PCR reactions must be performed sequentially from the N-terminus to the C-terminus.
pLPCX-Cx43-IRES-GFP was obtained from Addgene (#65433). pcDNA3.1/Hygro(+)-GJC1 (Cx45; cloneID: OHu04829) and pcDNA3.1/Hygro(+)-GJA4 (Cx37; cloneID: OHu33346) were obtained from GenScript.
Mulkearns-Hubert E.E., Torre-Healy L.A., Silver D.J., Eurich J.T., Bayik D., Serbinowski E., Hitomi M., Zhou J., Przychodzen B., Zhang R., Sprowls S.A., Hale J.S., Alban T.J., Berezovsky A., Bell B.A., Lockman P.R., Jha B.K, & Lathia J.D. (2019). Development of a Cx46 Targeting Strategy for Cancer Stem Cells. Cell reports, 27(4), 1062-1072.e5.
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2

Engineered Connexin Expression Constructs

Check if the same lab product or an alternative is used in the 5 most similar protocols
The Cx46 expression vector was created by inserting the Cx46 cDNA (catalog# RDC0535, R&D Systems) between the HindIII and XbaI sites of pEGFP-N3, excising the GFP tag. This backbone was used for site-directed mutagenesis to introduce the L11S, T19M, and cysless mutations, using the primers shown in Table S2. The primers for the cysless mutant were designed so that the PCR reactions must be performed sequentially from the N-terminus to the C-terminus.
pLPCX-Cx43-IRES-GFP was obtained from Addgene (#65433). pcDNA3.1/Hygro(+)-GJC1 (Cx45; cloneID: OHu04829) and pcDNA3.1/Hygro(+)-GJA4 (Cx37; cloneID: OHu33346) were obtained from GenScript.
Mulkearns-Hubert E.E., Torre-Healy L.A., Silver D.J., Eurich J.T., Bayik D., Serbinowski E., Hitomi M., Zhou J., Przychodzen B., Zhang R., Sprowls S.A., Hale J.S., Alban T.J., Berezovsky A., Bell B.A., Lockman P.R., Jha B.K, & Lathia J.D. (2019). Development of a Cx46 Targeting Strategy for Cancer Stem Cells. Cell reports, 27(4), 1062-1072.e5.
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