Anti gpr15 clone sa302a10

Following washing in FACS buffer, PBMCs were stained with anti-CD3 (clone UCHT1, BD), anti-CD4 (monoclonal, clone Sk3, BD), and either anti-GPR15 (clone SA302A10, BioLegend, San Diego, CA, USA)) or a mouse IgG2a kappa isotype control (clone MOPC-2710, BioLegend). Samples were resuspended in 100 μL of FACS buffer for staining and were stained with 1 μL of each antibody as per the manufacturer’s recommendations. Samples were acquired on a Becton Dickinson LSR II flow cytometer using FACS Diva Software. Fully stained PBMCs were compared with unstained samples and samples stained with IgG2a kappa isotype control. The percent of CD3⁺CD4⁺ T cells staining positive for GPR15 (hereafter “GPR15⁺ T_h cell percent”) for each subject was visualized and calculated using FlowJo software version 7 (Tree Star, San Carlos, CA, USA).